Appendix. 



DIRECTIONS FOE OBTAINING AND APPLYING THE CHINCH-BUG 



INFECTION. 



In order to keep up our supply of infected bugs, we must require that 

 each person sending for infection send us live bugs from the field. These 

 should be put into a tin box (a baking-powder box is excellent for this pur- 

 pose), without soil, and with a supply of green wheat or corn. The box 

 should have a tight-fitting cover, and no holes need to be made. We have 

 found that the bugs reach us in the best condition when sent in this way. 



CHARACTER AND LOCATION OF INFECTION BOX. 



Make a box of seven-eighths inch material (matched white pine is best), 

 about 24x36x6 inches. Make the cover tight fitting, so that the bugs can- 

 not readily escape. Weather strips tacked about the upper edges of the 

 box, so that the cover presses tightly against them when closed, will be 

 found of service in keeping the bugs in. 



It would be well for each farmer to have two boxes, and, after the first 

 box has become well started, he should start the second from infection ob- 

 tained from the first. 



Place the boxes so as to exclude them from the sun and wind — a base- 

 ment room is a good location. 



TO START THE INFECTION IN THE BOXES. 



Sprinkle the boxes thoroughly with water inside and out. Place an even 

 layer of garden soil in the boxes, half an inch deep. The soil should be 

 free from leaves or anything liable to decay. Sprinkle the soil until it is 

 moistened through, but not muddy. Put a thin layer of green wheat or corn 

 over the soil in the boxes, and distribute over this, at regular intervals, five 

 or six of the white-fungus-covered bugs. Now put into the boxes healthy 

 bugs from the field until they are somewhat crowded, but not packed over 

 one another. Lastly, close and fasten down the cover. 



Examine the boxes daily and sprinkle the sides and soil as often as they 

 appear to be getting dry. Do not, however, make the soil muddy. As soon 

 as the white-fungus-covered bugs begin to increase in your boxes, you may 

 commence to gather them for the infection of your field, but not before. 

 You should be able to do this in three or four days. Always leave a few 

 fungus-covered bugs in your boxes for continued infection. Replace the 

 green wheat or corn as often as it becomes yellow, and keep the boxes re- 

 plenished with healthy bugs from the field. 



TO INFECT A FIELD WITH THE WHITE-FUNGUS DISEASE. 



After you are made certain by the increase of the number of bugs cov- 

 ered with the white fungus that the infection is working in your boxes, you 



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