71 



After 23 hours 11,700. 



Experiment IV. 



„ 48 „ 



72 



„ 247 „ 



„ 316 ,, 



At time of mixing 



After 5 hours 



23 



48 



72 



247 



316 



3,250. 



3,250. 



455. 



325. 



130. 



41. 



31. 



38. 



negative. 



1. 



0. 



Experiment V. At time of mixing 31,200. 



After 172 hours 9,360. 



„ 244 „ 825. 



Experiment VI. At time of mixing 325. 



After 172 hours 2. 



Experiment VII. At time of mixing 32,500. 



After 504 hours (water kept at 8°C. to 10°C.). 79. 



Experiment VIII. At time of mixing 325. 



After 504 hours 0. 



These results are fairly uniform. AVhen a large numher 

 of Bacilli are added to the water their presence may- 

 be demonstrated longer than in cases where smaller 

 quantities are used. Fourteen days would appear to be 

 the average duration in sea water incubated at 35°C., whilst 

 when kept in the cold their presence was demonstrated 

 on the twenty-first day. There appears to be no initial 

 or subsequent multiplication of the Bacilli. Between 40 

 and 70 hours after infection there is less decrease than at 

 other periods ; but there is no evidence of increase in 

 numbers of the Bacilli when grown in sea waters either 

 when incubated or at ordinary temperatures. 



On the whole the investigations which are summarized 



