18 



II. — The Action of Sea-water upon the Growth of the 

 B. typhosus. 



These results are fairly uniform. When a large number 

 of Bacilli are added to the water their presence may be 

 demonstrated longer than in cases where small quantities 

 are used. Fourteen days would appear to be the average 

 duration in sea-water incubated at 35*^0., whilst when kept 

 in the cold their presence was demonstrated on the twenty- 

 first day.* There appears to be no initial or subsequent 

 multiplication of the Bacilli. Between 40 and 70 hours 

 after infection there is less decrease than at other periods ; 

 but there is no evidence of increase in numbers of the 

 Bacilli when grown in sea-water either when incubated 

 or at ordinary temperatures. AVe do not think, however, 



* Dr. Cartwright Wood, however, finds that tlie typhoid Bacilli may 

 possibly persist for two months. 



