( 338 ) 



Baümann ^) have latterly come to the same result in an almost 

 similar waj'. 



Besredka ') on the contrary tried to separate the different strepto- 

 cocci from each other with the aid of the method of the combination 

 with the complement. The conclusion to which he comes, is, that 

 the "substances sensibilisatrices" present in his serum, are "rigoureu- 

 sement" specific; that the serum of a horse, immunized with the 

 streptococcus A, only contains the amboceptor A", which corresponds 

 with that special streptococcus. Thus he found this amboceptor A" 

 not only active against the streptococcus A, but also against other 

 races {JB, C), from which Besredka decides on the identity or at 

 least on the near relationship of the above mentioned streptococci 

 A, B and C. 



According to these results, some experiments have been taken by 

 me, to trace, in how far a separation of the different pathogenic 

 streptococci is really possible by means of the specific action of the 

 amboceptors. 



The anti-streptococcus serum, which I used, Prof. Spronck willingly 

 provided me with, for which I offer him my best thanks as well 

 as for his further assistance in my work. 



The above mentioned serum originated from a horse, which was 

 injected for a great length of time viz. from Jan. 1905 till July 1906, 

 with a number of specimens of streptococci and staphylococci of 

 different origin. These injections, which were subcutaneous, took place 

 weekly. The quantities used were gradually increased during the 

 first months ; whilst after that on an average 40 — 60 c.C. of a mix- 

 ture, composed of even parts of a culture in ascitic-bouillon of the 

 different streptococci and of a bouillon-culture of the staphylococci, 

 were administered. The mixture was twice heated for half an hour to 

 55° C. Strepto- as well as staphylococci originated directly from man, 

 without passage through animals. 



That the serum really possesses curative qualities is evident, not 

 only from observations in the clinical surgery, but also from experi- 

 ments upon animals. Rabbits, which were injected with a mixture 

 of strepto- and staphylococci, could be kept alive by administering 

 comparatively small quantities of the anti-streptococcus serum, whilst 

 animals used for controll died shortly after. 



The method, followed by me, is that of Bordet — Gengou '). For 



^) Munch. Med. Wochenschrift, 1906, N". 24, S. 1193. 



2) 1. c. 



3) 1. c. 



