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follows: no heniolyse in the first (least diluted) tube, a little hemo- 

 Ijse in the 2"^ tube, more and more hemolyse in tubes 3, 4 and 5 

 whilst in the sixth (most diluted) tube it was perfect. 



The same experiment was made with different other bacteria with 

 a similar result. 



It may be easily understood, that it sometimes occurs, that no 

 hemolyse is formed in the first of the tubes, used in the method of 

 BoRDET — Gengou, in consequence of a surplus of bacteria, as is seen 

 by the absence of the hemolyse in the second tube at the same time. 

 Without the controll-tubes, one might wrongly decide on the presence 

 of an amboceptor in the examined serum. 



In the first place an investigation was made, whether in the anti- 

 streptococcus-serum, used by me, an amboceptor was present against 

 some five streptococci used at the immunization. The result was 

 positive. After this, different other streptococci were investigated. 

 These streptococci originated directly from different diseases of man, 

 such as : scarlatine, cholecystitis, septicemia, febris puerperalis, angina, 

 and had not served at the immunization. Among these streptococci 

 there were some of patients who during their lifetime had been 

 injected with the same anti-streptococcus serum, but without success. 



The latter streptococci were cultivated from the blood or from the 

 spleen post mortem. Others were cultivated from patients with whom 

 the injections of the serum had had a very distinct curative effect. 

 It was therefore supposed that against the first streptococci no 

 amboceptor would be found in the anti-sti'eptococcus serum. 



The investigation however did not confirm this supposition. All 

 streptococci, no matter what their origin, shoioed a strong combination 

 with the complement under the influence of the anti-streptococcus serum. 



Keeping to the specific of the amboceptors, the conclusion of 

 Besredka ^) might be accepted, regarding all the latter streptococci as 

 identic or at least closely related to those used at the immunization. 

 Continued experiments with some pathogenic streptococci originating 

 from animals, have led to a different interpretation. A streptococcus 

 was used, which was cultivated from the lungs of a guinea-pig, 

 which had died spontaneously from pneumonia; further the well- 

 known streptococcus equi and a couple of other streptococci, which 

 were cultivated with the Str. equi from pus, originating from horses 

 suffering from strangles. Also against the latter streptococci, the 

 presence of an amboceptor in the used serum was an undoubted fact. 



Considering that the str. equi by its qualities, apparently from its 



1) 1. c. 



