( 'M ) 



possible to cjirn the <l(»\viiy suhstaneo on tlieiii and lo place llie 

 infected parts of leaves and slems in damp <>lass-l)()xes al 22" (*. 

 A^ain a beginiiijig grovvtli was noticeable after 24 lionrs. 



Tlie pure cnltnres on the malt-gelatine plates became moi-e and 

 more extensive, forming circles which after three days had diameters 

 of 8, after fonr days of 13 centimeters. 



By and by the malt-gelatine was peptonised and in a smaller or 

 greater number of places, near the margin moi-e than in the middle 

 of the circles, small, white, glossy points arose, which secreted tlrops 

 of a colourless, quite clear liquid, but which required no more tiian 

 J 2 hours to turn into black dots. These also continued the process 

 of drop-formation for some time, when after some further increase 

 in size they changed into shorter or longer, round or angular little 

 bodies, whicii clearly belonged to the class of sderotia. Having 

 grown more and more independent of the hyphac which al lirsl 

 occluded them, tliese l)lack bodies could now be removed without 

 danuiging them and they appeared to have reached a maximum 

 length of 10 millimeters and a thickness of 5 — 6 millimelers. 



The experiments on infection with parts of living lobaccoplanls 

 were all successful on condition that the place of inocidation was 

 ke})t very wet, e.g. by wrapping it up in very wet cottonwool or 

 some woodshavings steeped in water. The attacked tissues became 

 discoloured also here. 



From what jtrecedes we may infer that the fungus cultivaled on 

 malt-gelatine does not dilfer from that of the tobaccolields, which 

 was irrefutably j)roved later when tVom the sclerotia of l)oth the 

 same Sclerotlnla was obtained. 



It is worth mentioning that the myceliumcultures on the mall- 

 gelatine whicli had produced the sclerotium, had besides given rise 

 in several places to didl white, granulated spots, which microscopical 

 examination revealed to consist of T^^ clusters of flask- or cone-shaped 

 conidiophores, borne by erect or ascending hy|)hae and 2'"'. a number 

 of curious crystals pressed against the thread-shaped cells, partly 

 loosely spread, j)artly assend)led in clusters. 



The colourless conidiophores were high 12 1(5 »< and broail 



4 — 5ft and consisted of a cylindrical body tapering a lillle lo\vards 



the lower end, a thinner short neck and a spherical head, which latter 



Just slightly exceeded the neck in breadth and produced spherical 



colourless conidia of 2.5 ji diamelei-, w liicli were al lirst comiecle(l 



to short chains, but soon broke up and commenced a)i indi\idual 



existence. 



The crystals and other V)odies, often striated, not occluded in cells, 



4* 



