( m ) 



iiilmto, potassium uiti-itc, cliloi-idc, nitrate, [>hos})liate, sulphate, 

 carbonate of ammonia and ammonia. Ammonium nitrate gave tlie 

 best results. The other compounds showed little difference. Of 

 ammonia which was added in very small (juanlities, hardly anythiuii- 

 was assimilated. 



Of amido compounds, whicli are generally known as good sources 

 of nitrogen foi- fungi, glycocoll, asparagine, a^jtartic acid, alanine, 

 tyrosine and leucine gave good results in the present case also. The 

 nitrogen of urea, creatine, parabanic acid and uric acid has little 

 nutritive \alue. From the last mentioned substance also carbon can 

 be assimilated. 



Among aromatic comj)Ounds, only the nitrogen of ammoniumsalts 

 has any mitritive value: among the derivatives of pyridine only the 

 nitrogeji of the residue, not the carbon. To develo]) the fungus glycose 

 has consequently lo be added lo the nutrilive material. Nicotine, 

 being a free alcaIoi<I can serve as a source neithei- of nilrogen nor 

 of carbon. 



If assimilable caritou is j)resent, the niti-ogen is used from the 

 ammoniumsalts of oxalic, tartaric, citric and benzoic acids, least fi-om 

 ammonium succinate. 



b. CarhonaceoiiM fond. 



Of fatty acids oidy vei-y dilute acetic acid (0.050 %) has a nut rit i\e 

 \alue for carl)on. 



The polyacid alcohols are bad sources of carbon, as was shown 

 by an investigation with glycerine, erythrite, mannite, sorbite, adonile 

 and dulcite. Least satisfactory was sorbite and also glycerine, a good 

 cai-bon-food for many fungi, gave bad results here. Lactic acid in 

 very small quantities, was available as a carbon-food. 



Very differently behaved the sugars. As was already mentioned, 

 glycose comes first in nutritive value. Besides were studied : aral>inose, 

 xylose, saccharose, fructose, maltose, lactose, raffmose and melibiose. 

 Of all these only xylose and arabinose had any value as sources of 

 carl)on. In all other solutions only a ti-ace of groNvth was ol)served. 

 Though not widiout difficulty the fungus was able to derive carbon 

 from cellulose. On filtering paper wetted with the above-mentioned 

 nutrient solution, l»ut without glycose, a snow white, woolly mycelium 

 developed. Also from inuline carbon may l)e obtained. 



c. Nitrogenous and carbonaceous food. 



As mixed sources of carbon and nitrogen we must mention aspa- 

 ragine, asj)artic acid and alanine. The addition ofpotassium niti'ate 



