:-;38 ) 



The li\ flrocN aiiic acid was lilral(M| hv Likhk/s iikMIkxI : iii tlio fii'st 

 j)ei-i(»<l (»l' llic i'caclioii. \alii('s are foiiiid coiTcspoiKliiig- \\]\\\ lliosc 

 of ihe siiuai- (IcUM-iiiiiiatioii '). 



1 N. Aiiivjidaliii solution. 



4. 'IMiosc tal»l(^s show tlial llic iimmilaliilil v of llic cxpi'ossion iii 

 Ihc tliii'<l coliiiiiii is salisfacloi'v. In 11knki"s cxiMM-iinciils tlioso values 

 (lilfer Will lilli<' more than ihosc of ihc iiixcfliii action. 'I'o sonic 

 exi(Mil the (v\|icrini('iital crroi-s iiiav ccrtainlv lic allrihiilcd to Ihc 

 sciisiti\ ciicss of Ihc cniidsiii and pai'tlv also to the method followed. 

 'I'hc laltlc w illi Tammanns cxpci'iincnts proxcs this. The conslaiils 

 val•^ within father lar.u'c linnts hnl aLiree reasonahlv w itli an avcnigo 



value. 



5. If we now accept the hypothesis of the neL>'ali\e -) anjocata- 

 hsis. and after what has Ween stated this seems to me (piite ])ei'- 

 ]nissil»le. tlici-e w ill he fonnd to exist an e\ideiil parallelism Itetween 

 the action of emnlsin ') and inxcrtin. 



The lernienl-i-eaclions which np to the pi-esenl haxc been accni-alelv 

 stndie(l pi'oc(>ed Iherefoiv accoi-din.u to the scheme : 



'''^(/•^ =b /•.,..) (,/-.o (11) 



<lt 



in which l\. mav also he /.ero ')• 



'I'his, however, oiilv means that /• is constant fof the same sei'ics 

 of e.x[)erimenls or for a delinite concentration of material '' i and enzyme. 



We mnv sav that there exists a fnnction of the h»rm : 



/■,-/••("./') (12) 



in which d is the concentration of tin- inxv'.tai le mailer and A thai 

 of the feniienl. 



1) Compare Tamman.n, Zeilsclu-. liir pliysikali-sclie Clieniie. 3, -21 (ISSO). 



-) This may. 1)0 one of the cau.ses that tlie synlhctical fxpciiinents willi ciniilsin 

 (Tammann, Emmeuling) have given a negative result 



•■') This is probahly also tlie case witli other ferment-reactions. 



1) It is not inconceivable that cases may occur where if t is small, .r would al 

 hr.st act positively and afterwards negatively. 



■') Up to the present it is only haemase for which Senteh (Zeitschr. lïir |)liysi- 

 kaliscbe Chemie 44. ]». :2r.7, (l'.tOÜ)). lias ohiained (lin'ercnl rcsufs within ;i small 

 concentration limit. 



