( Bi)8 ) 



inosl ('oimnoii liimiiis-iiiliahilaiits IVoiii llic tbrcsl of Spaiidcrswoiide, 

 y)'/('//(i(/('i'/i/(/ /,-(>/i///(/f/\ |Kiss('ssos llic same |n-()|)('rly. 



Wc luxvc öiRTcedcHl ill (iiidiiiu- nii (v\|KMiiii('iit l>v nioaiis of wliicli 

 llic (•('lliilose-dosli'fniiiij,' moulds iiiav lx- isolaled from iialiiiv iii 

 a direct and c(M-|aiii manner. Two sici-ile discs of Swedish liller|»a|>er 

 are. |)laced in a .U'lass l)o\ and moislened willi tlie following' li(|nid: 



Tapwaler 100, XH^XO,, 0,05, KHJ^O, 0.05. 



As infecting- material o;w\\\ or litimns may he nsc(h hnl die hest 

 residts are ohtained hy simply e.\|)osin<>- the Oj)eiied box forahonl 12 

 honrs to llie open air. If then wcMMdtixale at "24 and take care lo keep 

 die paper moist, colonies of nionhls already i)ecome visible after 5 or 

 G days, hnt it is only after 14 days or three weeks, that we notice 

 the enormous richness of these cultures, and then we are surprised 

 at the great iiunihei- of monld species, which make their appearance. 

 iMany kinds which we seldom or never notice on malt-gelatin are 

 found in large iiumlters on these paper discs. These species certainly 

 are also ca,pable of gi-owing on mall-gelatin, but their germs, as 

 they occur iu nature, apiiarcnlly find thereon an unfavorable soil. 

 Another advantage of the cultivation on paper is that it is pariicn- 

 Jarly fa\'orable to the formation of perilhecia and picnidia. n\ liich 

 do not readily de\elo|) on ri(di soils M. 



From these culture expei-iments it a|»pears that a continuous rain 

 (d' spores from cellnlose-destroyiiig moulds falls in the garden as well 

 as iu the rooms of the bacteriological lahoralory. For instance on 

 March 11. when the wc^ither was dry whilst the eai'th was moist, 152 

 cellulose-deslroying monlds were collected on a plate of 275 cm. s(piare, 

 after this had l)eeii exposed for 12 hours to the open air, and among these 

 monlds about 35 species were recognised. As these germs mnsl con- 

 tinually drop on the soil, it might be expected that the latter woidd 

 1)1' remarkably rich in lixing nioidds and it appeared from experi- 

 ments, that this is really the case at the surface of the garden soil, 

 but in a much less degree than might hav(^ been expected, while 

 lower down iu the soil the number of moulds seems to be still less. 

 From this it follows that most of the spoi-es, w liicli fall on the eai-th 

 rapidly die oil'. 



In (U'der to prepare a pure cultuix' of the fungi isolated l>y the 

 "paiter-experiment" some material from the raw cultures was ti-ans- 

 ferred to malt-gelatiii, wheiv it ajtpeared that the moulds wei-e 

 generally much contaminated x\itli hacleria, frcnn which howe\-er. 



^) (!oinj);\re Molliauu, bül;' dt^.s bactriics <\m\> la iMiHlndion «Ifs iiriillièfcs des 

 AscoboUts, C. R. t. 13G, l'J03, p. 899. 



