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surplus was removed by dinatriumphosphate and the liquid then 
obtained reduced to a definite volume. In this two estimations of 
sugar were made, one before, the other after allowing emulsine 
to work in upon it for 48 hours. Prefatory experiments with pure 
salieine had proved that in this way it was completely decomposed: 
the increase of the reduction after inversion was to be attributed 
only to the glucose formed of salicine. *). 
From this increase of the glucose the quality of the salicine could 
then be calculated. 
This same method was followed in order to state the salicine in 
various parts of the plant; then however, after inversion the liquid 
was extracted with ether, so that saligenine might enter into it. This 
substance is easily recognised by the physic qualities of its crystals 
and by the substitute of bromine obtainable with brominewater and 
moreover by its salt of copper. The efforts to point out salicine in the 
tissue itself were unsuccessful; the method formerly used by THrORIN ©), 
namely that of adding concentrated sulphuric acid, proved impracti- 
cable, as it during the produced erroneous results. 
For the above mentioned Salix species salicine is found in the 
bark of the branches, but not in the wood; young buds are rich in 
it, likewise the assimilating leaves. It appears in young ovaries 
but disappears during the process of ripening. 
Although an inverting enzyme was not to be extracted, it proved 
necessary to kill the parts immediately in boiling water, otherwise 
considerable alterations in the quantity of salicine presented them- 
selves. Thus e.g. after slow drying 25 pCt. disappeared out of 
the bark. 
The following series of determinations for the purpose of invest- 
igating the quantity of salicine during the budding period, was made 
with one specimen to exclude individual differences. 
The total quantity in various successive stages was calculated by taking 
a branch with a definite number of sidebuds as object. The weight of 
the different parts of this branch together with the procentie values 
of the quantity of salicme in corresponding parts of the same object 
in the successive stages gave the total quantity of salicine of this 
branch in those stages *). 
1) Before inversion a solution of salicine does not reduce even with boiling ; neither 
does saligenine formed by means of inversion at the same time as glucose. 
2) See Theorin Ofversigt af Konel Vetenskaps. Akademiens Förhandlingen 1884. 
No. 5. Concentrated H, SO, gives with salicine a coloring of red. 
3) In corresponding parts of one object was an equal quantity. 
