( 298 ) 
These quantities are small compared to the entire quantity consumed 
+ 330 mG. for 100 young shoots. 
When the young buds were budding forth saligenine was found 
in them, the branches were immediately killed in boiling water, the 
extract after cooling down extracted with ether; so all influence of 
enzyme could be excluded. It becomes very probable that the salicine 
is analysed before the consumption, on account of saligenine being 
found ; the quantity, however, is so small that if really the analysis of 
salicine were to take place as indicated, and a decomposition to precede 
the consumption, saligenine can only be an intermediate stage. Hither 
the aromatic half disappears as such, or another aromatic substance 
must be the definite product of the decomposition. 
In the young leaves developing normally, salicine soon makes its 
appearance again after having disappeared for a moment; we can 
expect that this increase is connected with and due to the assimilation, 
as etiolated shoots do not show it. In order to state whether the 
leaves were really the place of a new formation and the light really 
had a part in it, the quantity of salicine before and after darkening 
was compared. 
The quantity in the leaves was determined in the evening after 
sunset and in the morning before sunrise (one specimen). Likewise 
in the evening leaves were halved, one half with midrib left on the 
plant, the other half analysed. The following morning the remaining 
half was cut off from the midrib and also analysed). Provided that 
a sufficient number, 100 or 200 leaves were halved, a comparison 
could very well be made. 
For a small-leafed specimen a 100 leaves 
8 P. M. 7 Aug. 47.5 mG. glucose 87.2 mG. salicine 
EAM B = eee " 60.2 / Ĳ 
For a big-leafed specimen a 100 leaves 
8 P. M. 7 Aug. 80 mG. glucose 177.7 mG. salicine. 
4 A MESS) ein ed, DE ENA TT 
So in both cases we see a decrease during an 8 hours’ summer 
night of respectively 30 and 20 °/, of the salicine in the leaf in 
the evening. 
For experiments with entire leaves of one specimen : 
8 Po M7 Aug. 467% salicine: 
4 As Mas Sut) iy ea rde Ee oe 
B ACME Br Mi Aen Petr 
1) See Lotsy. Mededeelingen ’s Lands Plantentuin XXXVI, 
