( 301 ) 
above). For this then a CH, group out of the lateral chain would 
have to be decomposed, as saligenine is orthoöxybenzylalcohol and 
catechol is the orthodiphenol. 
Corresponding to this the quantity of catechol of the bark is large 
in May(1.1 pCt), a greater part of the salicine then having disappeared, 
much lower in July (0.3 pCt.) when the loss has been repaired *). 
Where now has the decomposition taken place ? 
PrerFer says Kap. VIII, Pflanzenphysiologie 2: Auflage: „vielleicht 
dienen die esterartigen Verbindungen der Kohlenhydrate mit Phenol- 
körpern zur Herstellung von schwer diosmirende Verbindungen bei 
deren Zerspaltung im allgemeinen der Phenolkörper in der Zelle 
intact verbleibt, um fernerhin wieder zur Bindung von Zucker benutzt 
zu werden.” 
The facts are excellently explained in the following way: 
The decomposition of the salicine takes place in every cell, the 
glucose is conveyed in the direction of the green parts, the catechol 
remains in the cell and binds glucose, coming from cells situated 
closer to the bark, to salicine. 
Glucose is transportmatter and salicine is transitory reservematter. 
The glucose being comsumed in young parts in greater quantities 
than its supply is, catechol must be found, but only so much as 
corresponds to the decrease of the absolute quantity of salicine. 
100 young shoots 18 m.M. long 28 m.G. salicine, traces of catechol. 
ROO 5 i SO. tM ght) 216 mG: i 2 m.G. " 
6.4 m.G. salicine corresponds when calculated to 2,5 m.G. catechol, 
when observed to 2 m.G. 
This correspondence adds great strength to the hypothesis. *) 
In the bark the loss of consumed glucose is not repaired, so 
catachol increases greatly. 
As for Aesculus, here it was especially the germination which 
was studied. The glucosides found in the ripe seedlings being not 
yet chemically determined, it was only necessary to base the method 
of the quantitative definition on the quantity of sugar formed by 
inversion. I had to trace whether the quantity of sugar bound in 
glucoside decreased during the germination. 
To this end the seedlings were ground and extracted with methyl- 
alcohol, of this extract the alcohol was evaporated, and the watery liquid 
1) [| here mean the quantity in the bark of thicker leafless branches where no 
difference between night and day is observed. 
2) Also the facts observed at the change of night and day can be excellently 
explained in this way. 
