( (oa), 
extracted with ether to get rid of oil and resin. The extracted 
liquid served as definition of the reduction before and after inversion 
by boiling it for 2 hours with HCl‘). ; 
From the difference of this reduction the quantity of reducing 
sugar originating from the glucoside could be calculated; it amounted to 
13 pCt. 
During the germination this quantity decreased in cotyledons by : 
60 or 70 pCt. Fecula and albumen by 70 or 80 pCt. The germinating 
plants contained only 1 or 2 pCt. of glucose bound in the shape of 
glucoside, the consumption of the glucosided sugar during the germina- 
tion could be regarded as proved by the 70 pCt. decrease of the 
absolute quantity. 
The localisation of aeseuline was observed by fluorescence of its 
watery solution, to be seen when there are not too few sections. 
Aesculine was to be found in ungerminated seeds only sporadically 
in the plumule; when germinating it appears in greater quantity in 
the stalks of cotyledons, not in the cotyledons themselves. Stalk and 
hypocotyledon internodium contain aesculine when germinating in the 
dark as well as in the light, so light is not necessary for the formation. 
The stalks of the leaves show the aesculine only when developing in 
the light and not in the dark; this seems to point to the fact, that the 
aesculine of the normal germinating plant originates from two sources : 
that it is formed for the greater part by reforming of substances out of 
the cotyledons and side by side with this, that it is prepared inde- 
pendently in the stalks of the leaves from substances assimilated by the 
leaves. Experiments with full-grown planis, in the light and in the 
dark, with coloured and with normal leaves made this the more 
propable, but full certainty can only be given by means of later 
quantitative definitions. 
Studies on Gaultheria procumbens showed what changes took place 
in the quantity of the gaultherine, the investigations have however not 
yet been brought to an end. The method of quantitative definitions 
was founded on the observation of the quantity of methylsalicylate 
Which could be formed out of it. This was redistilled with vapour 
out of the parts, caught in alcoholic potash and saponificated with it. 
The kaliumsalicylate formed in this way was determined according to 
the method of Mrssincer and VorTMANN ®). For smaller quantities the 
colorimetric method of determination was used with Fe Cl, 
1) After inversion and neutralisation the liquid was treated with leadacetate, 
2) Messincer and Vortman, Zeitschrift f. Anal. Chem. 38 bl. 292. 
Ber. d. deutschen chem. Geselischaft. Berlin. Bd. 22. 2313, 
