( 166 ) 



It is perhaps also possible to explain the phenomena in an othei- 

 waj. It might be assumed, that onlv one knot of similar ti bres con- 

 nects the heai-t with the bulb. If the heart were exposed to no 

 other influences, the stimulation of these fibres would always have 

 the same effect, i.e. a decrease of the frequency of the heart beats 

 or a standstill of the heart. But we have to take into consideration, 

 that as a matter of fact in normal conditions there are still other 

 influences which affect the heart, e. g. those, which are brought 

 about by the accelerator nerves. And the variations in the excitatoi-y 

 state of these nerves might perhaps produce all the ditferences in 

 the effects of vagus stimulation. 



This explanation, which in our opinion is a less probable one, 

 can be tested, if our experime]its are repeated with this ditfei-ence, 

 that previously the rm. accelerantes are sectioned. 



The detailed account of our investigation >vill be published elsewhere. 



Microbiology. ■'Thi')iii()-t<)hT<i})t ///>iis('''. Vn Dr. X. L. Sohngkn. 

 (Coninniuicated by I'lof. S. Hoookwkh fk). 



Thermo-tolerant lipase means in this communication a fa1-sj)lilting 

 enzvme able to resist a temjierature of 100° ('. during 5 minutes 

 without being decomposed. 



Thermo-tolerant lipase is secreted by the microbes of the group 

 of B. fluoresci'ns Uqwfndëns, to which in this relation are also 

 reckoned B. pimctafum, B. pf/oci/iwum and B. Uffiefaciëiis alhiis. 

 The enzyme is not formed by the ^voup oï B. lipofj/ticuni, B. .shdceri, 

 B. fluorescent non-Iique/nciëns, neither by Oïdliun Incus, /Upergilbis 

 ni(/er, PoüciUluin (jlaticum, and Cladosporluni hutijri, all of which 

 secrete a fat-splitting enzyme which decomposes already at 80° C. 



The most convenient medium for the above melting bacteria is 

 broth with 3 ", „ peptone, the cultivation being successfully effected 

 in Erlfamkyki{ flasks under aerobic conditions at + 23° C. 



When such a medium is inoculated with one of the said melting 

 bacteria the microbes will after six days' cultivation have secreted 

 a considerable quantity of lipase so that the culture liquid in a 

 fatted tube at 30° C. shows the lipase reaction already after 1 hour. 



The following investigations are made with cultures aged 6 — 10 

 days. 



Experiments on the decomposition of the microbic lipase by influences 



