( 1B9 ) 



(/Onseqiioiillv llic lipaso is not (lc('ornp().so(U\lioii hoilcd (ni(> iniimie ; 

 this is the case after 5 minutes' hoiiiiiii'. 



It is notewortli}' that the <|iiaulitv ol" Cat splilted I)y iioii-heated 

 and l)v l)()iie(l cidtures is the same, from which faci il ma\ i»e 

 inferred that the mici'obes of tlie .<>r()np of meltinji' Hiioresccnts prochicc 

 only one lipase, namely thermo-tolerant lipase. 



Determinafio)) of the rc/oc/'fi/ of thr (Iccoinposifion of lijxisi hif 

 IwatiiKj at 99°- 100° C. 



These experiments were effected Jiisl in the same; \va\ as those 

 wilh l)oile<l lij)ase on 1 



(^)uanlity of falty Decoloration 



7 June 1911 acid formed, (wpressed of the 



in cM". V,„ N. faded lidies 



Consequently thermo-tolerant li|)ase resists heating at 99°— 100° C. 

 dui-ing 5 minutes without losing a c(jnsidoral)le part of its activity. 

 If the heating is prolonged the xelocity of the decomposition increases 

 very much so that after 25 minutes' heating the enzyme is quite 

 destroyed. 



Influence of acids on. tJw /(tt-sp/d(/n</ hij tlu'fiiw-tolefaiit lipase. 



From a series of investigations conducted in the same way as 

 those with ordinary li|)ase described in a previou.s communication, 

 followed that thermo-tolerant lipase is still more sensible to acids 

 than the former. Already in a medium of \/,„|, N acid the enzyme 

 splits hardly any fat, whereas ordinary lipase splits still feebly in 

 a Vso ^ ^^^''f' liquid. 



Neuti-alisation of an acidified thermo-lolerant lipase culture renders 

 the enzyme again acti\e; but when the thermo-loleranl lipase is 

 boiled in a \ ery feebly acid medium the enzyme is quite destroyed. 



