( 312 ) 



12.30 p. m. 350 gr. potatoes, 50 gr. rice, 20 gr. butter, 100 gr. 

 sugar, 300 cc. milk, 300 cc. water, 

 7. p. m. 150 gr. wheaten bread, 50 gr. cheese, 20 gr. butter, 

 300 cc. milk. 



During the rest of the day he took neither food nor drink. 



From June 20 to July 5 the urine was collected in 3 portions, 

 from 8 to 3, from 3 to 10 and from 10 to 8. From July 6 till 

 July 19 the morning-portion was divided into one of 8 to 12 and 

 one of 12 to 3. In each portion the content of creatinin was stated 

 after the method of F'olin by means of the colorimeter of Vat^ 

 HooGENHUYZE aiid Verploegh, first in the unchanged urine and next 

 after the urine -|- 2 Vol. n. HCl had been heated at 115° C. for half 

 an hour in order to ascertain whether an}' creatin could be found. 

 The total output of nitrogen was stated after the method of Kjeldahl. 



Six times, viz. on June 30, on July 3, on July 9, on July 15 

 and on July 18, each time from 8 to 12 a. m. the muscles were 

 kept in tonus as much as possible. In order to compare this to the 

 influence of muscular labour Mr. Harkink took a walk of 20 K.M. 

 from 8 to 12 a.m. on July 12. On June 20 he weighed 72.5 K.G., 

 on June 29 73, on July 10 71,7 and on July 20 72 KG. 



The examination of the urine proved that on the days of the 

 tonus more creatinin was excreted than on other days. On the other 

 hand the performance of mechanical work — a four hours' walk 

 over against a four hours' tonus, which was kept up as much as 

 possible, yet not continually — had no perceptible influence on the 

 amount of creatinin excreted on that day, in accordance with the 

 statement of Van Hoogenhuyze and Verploegh. Also on the day after 

 the tonus the creatinin-figure was every time comparatively high 

 and no wondei'. For creatin introduced into the circulating blood is 

 not immediately nor entirely removed in the shape of creatinin, as 

 far as it is not decomposed, as experiments on animals have shown. 



The average excretion \)er day was: 



normally (J 8 days) 1.493 (max. 1.527, min. 1.361) mgr. 

 tonus (6 days) 1.614 ( „ 1.640 „ 1.573) „ 



next day (6 days) 1.525 ( „ 1.545 „ 1.454) „ 

 walk (1 day) 1.534 mgr. 



The minimum of the normal days regards the flrst day of the 

 whole series of experiments. For the rest it never fell below 1 .444 mgr. 

 Creatin was not once found in the urine. 



On the tonus-days there soon was an increase of the excretion. 

 This was especially evident, when from July 6 onward, the urine 



