( 373 ) 



of reducing sugar and organic acid at (lie beginning of the experinnent ; 

 the latter in the experimeiits with the alcoliol precipitate from tlie 

 spadices amounted to 0,1 cc 0,1 N at most. 

 Below are given some of the experiments. 



I. Experiment in CO, free air. 



400 mg. crude enzyme, acetonic preparation. 

 Decrease of glucose from 437 to 360 mo-. := 77 mo-. 

 Increase of the potash bulbs by 00, production 34 mg. 

 Increase of organic acids 9 c.c. 0.1 N. 



II. Experiment in CO3 free air. 



750 mg. pressed and dried tissue powder, that had been 



treated with acetone. 

 Decrease of glucose from 457 to 217 mg. = 240 mg. 

 Increase of potash bulbs by CO, production 140 mg. 

 Increase of organic acids 25 c.c. 0.1 N. 



The glucose can be more strongly decomposed, so that after boiling 

 wdth HCl only traces of reducing siigars remain ; in these experiments 

 K2HPO4 was generally added, which according to the investigations 

 of Zaleski and Reinhardt ^) stimulates the action of the enzymes. 

 This made a determination of organic acid difficult, because the 

 potassium phosphate had an acid reaction and the quantity added 

 perhaps partly entered into combination during the experiments *) 



III. Experiment in H atmosphere, potassium phosphate added. 

 800 mg. crude enzyme (alcoholic preparation). 

 Decrease of glucose from 437 mg. to 2 mg. = 435 mg. 

 Increase of potash bulbs by CO2 production 165 mg. 



IV. Experiment in air free from CO2, potassium phosphate added. 

 1.500 gr. pressed out material, which had first been dried at 



60° C. ; then acetone had been poured over it and it had 



again been dried at 60° C. 

 Decrease of the glucose from 485 mg. tot 5 mg. = 480 mg. 

 Increase of potash bulbs by CO. pi-oduction 187 mg. 



Finally two more experiments with the crude enzyme from the 

 leaves ; no potassium phosphate was added. 



1) W. Zaleski and H. Reinhardt Biochem. Zcitschr, Bd 27. 1910. 



2) Compare the inve.stigalious of A. Harden and W. J. Young. Biochem. Zeitschr. 

 1911. 



