( 379 ) 



ill corrosive alcohol of Schaudinn ; washed in water, alcohol and 

 iodine, hardened in absolnte alcohol, nionnted in j)arafüne, and cut 

 into sections of 3—5 [i, which where coloured with haeniatoxyline 

 of Ehrlich, Delafikld or Hridenhain or with Giemsa's solution. In 

 order to discover polar filaments living spores were examined. With 

 none of the reagents nsed (canstic soda and potash, snlphuric-acid, 

 hvdrochloric acid, nitric-acid, methyl-alcohol, ether, iodine, distilled 

 water), was it possible to demonstrate a polar filament, 



3. The Ufe-liistorij of Pieistopliora glgantea. 



The yonngest stages of the development of the parasite that I 

 could discover, are uni- or multi-nucleate cells 9.8 — 11. 2 ft long 

 and 4.6 — 6.3 n broad, situated between the connective tissue oi 

 the tnmour (fig. 1). Probably these cells mnltiply by schizogony, 

 it seems at least that stages as those of fig. 1 point to this fact; 

 they are each surrounded by a thin membrane. These cells represent 

 evidently the \egetative stages of the development, they are the 

 trophozoites. In the beginning these cells are quite diffnsedly spread 

 between the fibrils of the cellnlar-tissne. Afterwards they unite into 

 smaller or larger groups (fig. 2) and surround themselves at last 

 with a membrane, which, thongh very thin, is after all thicker than 

 that of the separate individuals. 





Fig. 3. 



In the individuals that have united into groups and are surrounded 

 by a cyst- wall (the pansporoblasts) the nuclei range themselves 



26 

 Proceedings Royal Acad. Amsterdam. Vol. XIV. 



