( ^«j ) 



llic inotlicr cell, and with it at the same time one of the groups of 

 two nuclei. 



The cysts formed in this way containing the bi-nncleate sporo- 

 blasts are of very different sizes. The smallest have a diametei- of 

 11 — 49 (I, the largest which usually contain only ripe spores and 

 show only at the periphery a layer of sporobJasts (tig. 6 rejn-esents 

 part of the periphery of such a cyst), can become 0.49 by 0.86 mm. 

 to 1.44 by 3.82 mm. and even reach still larger dimensions. 



By the formation of a thick cyst-wall the sporoblasts, i.e. the round 

 bi-nucleate cells change into the real spores, which have also 

 two nuclei. Besides the bi-nucleato spores some are found with 

 a single large nucleus, so that it is probable that the latter 

 is formed by the fusion of the two sporoblast-nuclei. If this 

 were the case, then we should have to regard this phenomenon 



Fig. 



as an autogamy. The spores are 5 — 6 ji long and 4 — 5 ft broad. 

 Besides the single or double nucleus no other organs as polar capsules 

 and the like are to be observed in the plasma ; in and beside the 

 nucleus however sometimes chromatoid granules are found, which 

 show in their microchemical reactions distinct affinity to volutine. 



As I remarked before the spores formed in this way are united 

 into smaller or larger cysts. Besides these however spores are found 

 that are diffnsedly spread between the connective tissue. It is likely 

 that these spores originate in trophozoites that were not united 

 into groups and surrounded by a cyst-wall. As T have however 

 never been able to observe the formation of these diffnsedly spread 

 spores in particulars, I cannot decide with certainty on this point. 



The reactioii of the hosts tissue against the parasitic in\asion is 



2G* 



