1182 



and by blowing out its contents rapidly and then blowing through 

 air for a moment, the potassium carbonate solution was mixed 



cztk^ 



Fig. 1. 

 within a few seconds with the liquid in the reaction tube, stopping 

 the enzyme-action abruptly. 



The tube A was then connected with the air-supply and the 

 ammonia blown over by a vigorous current of air, washed through 

 sulphuric acid. Two hours was proved to be amply sufficient for 

 the quantities of liquid used. 



Larger volumes would have been difficult to handle. 



In order to obtain sufficient accuracy in estimating these very 

 small quantities of NH, single determinations were not sufficient. 

 On two consecutive days identical series of experiments were carried 



