1225 



If the glass dishes have good dimensions and the space Lr is not 

 too small, one can sideways look through the glass wall and follow 



'^y//yyy/yy////////yyyy^^^^^^ 



Op 



-9 

 -Gs, 



Cultivation of anaerobes by means of Oidium lactis. Gs^ large 

 glass dish with the oxygen-absorbing Oidium plate Op. Gs.i smaller 

 dish with the culture plate Ka whereon the anaerobic colonies Ak 

 grow. Lr space between the plates. At g the hole in the glass wall of 

 Gso for the escape of the air from Lr, which is afterwards closed 

 with paraffin. Gd glass lid of the large dish Gsi- The higher 

 temperature is at the side of Gd. 



the development of the anaerobic colonies on Ak. So it is easy to 

 decide when liie moment ïov further observation has come without it 

 being necessary to remove plate Ka from the Oidmm plate Op, 

 and thus prevent too early opening. 



When it is time to open, liquefied malt agar must be at hand to 

 be poured out over the Oidium plate, especially in the groove 

 formed by Gs^, as soon as plate Ka is to be restored to its place. 

 The fresh food causes new oxygen absorption by Oidium and the 

 growth of the anaerobes can go on. 



For the success of the experiment it is essential to mind the 

 following. The |)lacing in the incubator should be managed in such 

 a way that the Oidium layer Op comes in the coolei, and the 

 cover Gd as also plate Ka in the warmer part. The vapour in Lr will 

 then condense in Op and not on the surface of Ka. In the reversed 

 position Ka will become moist, the colonies intermingle and the 

 experiment fails. Hence the figure is represented in such a position 

 that the colder air is above, the warmer below, as is the actual 

 state in an incubator with bottom-heat. How simple all this may 

 appear, in tiie execution if will be found necessai-y to pay special 

 attention to it. 



