1226 



In this way it is possible from the oidiuarj crude bntyric-acid 

 fermentations, obtained as described above, to separate tliree distinctly 

 different Aitiylobacter species, two of which I described alieady 

 before (Proceedings Vol. 12, Pag. 973, 1903) under the names A. 

 (Granulobacter) saccharohutyriciun and A. (G.) pectinovonwi, while 

 from the butyl-alcoholic fermentations two species were isolated, one 

 of which produces large slimy colonies and was described as 

 A. (Gr.) buti/Ucum (Archives Néerlandaises, 1"" Série, T. 29, Pag. 2), 

 whereas the other, which secretes no slime, has not yet been 

 investigated. The colonies of all these species colour dark blue with 

 iodine like starch, the slaves and clostiidia containing a great 

 quantity of granulose. 



The butyric-acid and buiyl-alcoholic fermentations acquired in 

 other ways than the above mentioned have not yet been examined 

 thoroughly. 



As the anaerobic Sarcinn ventrlculi likewise develops very 

 well on malt-infusion agar at 30"^ tot 37° C. (Proceedings 28 April 

 1911, Pag. 1412), this SF)ecies may be isolated just in the same way 

 as the above. 



As regards the spore-producing bacteria of the real protein putre- 

 faction the (>/(//^////-plate may be prepared just as in the experiment 

 described, only for the cultivation of the anaerobes themselves in 

 Gs^ it is better to make use of broth agar with 0.5 or 1 Vo common 

 salt, either with addition of 2 7o gincose or not. In this case, too, 

 nutrition with carbohydrates gives in some species rise to production 

 of granulose, in others luit. 



Another anaërobe isolated by the Oidmin-me\\\o(^ is Bacillus acidi 

 urici (Proceedings 23 April 1909, Pag. 990), which ferments uric 

 acid to carbonic acid, ammonium acetate and ammonium carbonate 

 This species also develops best on broth agar at 30° to 35° C. 



For beginners it must be noted that on plate Ka the facultative 

 anaerobes, such as Bacterium aërogenes and B. coli, develop quite 

 well, as may be proved by streaking off all the colonies Ak on 

 aerobic plates on which the anaëiobes only do not grow. This is 

 in accordance with the fact that at the starting of the experiment 

 some oxygen is present in Lr sufficient for the very small oxygen 

 want of the facultative, better called temporary anaerobes. 



