1318 



The uinmoiiirt in the original 175 c.c. solution was estimated in 

 tlie same way as well as the small quantities, whicii might have 

 been blown o\ei' with the CO2 in the controlling tubes. 



The results are represented in table 15 and in fig. 5, in which 

 for the sake of comparison also the straight line and the logarithmic 



curve foi- loq = //, are drawn, both through the origin and 



the first point, determined for //• 



TABLE 15. 



3 gr. of Soja on 286 c.c. 



ammonium-carbonate (= 2 Oq urea) 



nc 

 0.5 "0 urea />// = 7, hence ^ 01 



/(minutes) y ' ^i-y' '-^ ^ \-y 



m^ / * = ~7" 



i 

 45 



90 



135 



165 



180 



195 



210 



Repealed on manv different ways these experiments always produ- 

 ced similar results. 



For instance: Febr. 3"', 1916. 3 g. of Soja extracted with 200 

 c.c. of ammonium carbonate solution. 



A row of test tubes, each with 10 c.c. of filtrate and one drop 

 of octylalcohol in the bath at 27°. Each tube connected with a 

 wide tube (over the rim of the bath), containing 25 c.c. of 

 H,SO^ f N and some water. Hence current of CO, first passing test- 

 tube anil then wide absorption-tube. 1 c.c. of a 2,75 '/o solution of 

 urea, out of flask in the same bath, added to each test-tube. Reac- 

 tion stopped, without opening tubes or loosing connections, by 

 running 25 c.c. of saturated potassium carbonate solution into test- 

 tubes and blowing over the ammonia during the whole Jiight. Next 

 day titrated directly in the wide tube with NaOH ^V N and sodium 

 alizarin sulphonate as indicator. Two test-tubes with 10 c.c. of 

 extract, without urea, treated in the same way. 



