806 
9th Serves of experiments. 
a. 10 ce. ¢. of phosphoric acid, 18 ce. ¢. of sodium hydrate and 
27 ec. €. of water were mixed at room-temperature with a mixture 
of 25 ¢. c. of enzyme R+ 25 ec. c. of water. We examined directly 
the activity of this mixture, in which the enzyme had been diluted 
four times. It was subsequently warmed to and maintained at 37°, 
while at various intervals the action was noted, every time by 
allowing 2 ¢. ec. to act upon mixtures of phosphate and amylum of 
the optimal reaction. 
Time (minutes) durin | se ; | 
Ne uch SLMS eC NM (derne 
| 
1 joe 177.35 194.3 | 6.06 
2 8.75 | 179.10 — | — 
3 16.75 179.10 193.0 | 6.00 
4 4 179.10 AE ee 
5 88.75 179.10 | 193.0 6.075 
6 178.75 181.60 aed == 
7 268.75 | 179.10 193.0 5.975 
The pa of the enzyme-mixture was 5.502. 
b. 10 ee. of phosphoric acid, 12 ec. c. of sodium hydrate, 28 
c. ce. of water. Addition: 25 c. ce. of enzyme R+ 25¢. c. of water, 
amylum solutions as in the preceding test; py of the enzyme-mix- 
ture 4.095, 
Reduction Rotation | 
m.gr. Cu, | minutes Pry 
_ Time (minutes) during | 
Nr. ‘which the enzyme-mixture, 
was maintained const. at 37° 
1| 0 155.00 201.0 5.98 
2 18 147.85 201.7 | 6.04 
3 | 47.5 139.70 199.0 6,02 
fresh enzyme-mixture made 
of the same Enzyme Rand 
the same Pj. 
| 0 162.25 | 199.3 | 6.03 
5 | 138 I 413.00: | - 20125 608 
6 313 56.30 203.0 6.03 
