1049 
TABLE I. ‘FIXATION OF NITROGEN BY ASPERGILLUS NIGER. 
50 cm3. tapwater !), in which dissolved 0.15 0, NH4NO3®), 0.15°%9 KHgPO4, 0.06 "/o 
MgSO, (free from water) with the organic food given below. Temp. 33° C. 
Nr. - Organ. food Age By Ms inthe Boud Particulars 
(in mgrs.). 
1,2,3| 2 gr. glucose 5 to 6 months | Eras Vis; Jay 
4,5 2 „ levulose ato) (6 i 16.5; 18.6 
6 | 1 „ glucose 5 5 | 9.8 The culture liquid 
| | kn ä 4 to 5 * | 9.7 contained besides 
a enn | 4 EE ech considerable quan- 
9, 10 | hi . 3) | 4 PY | te) CHK > | tities of ammonia 
I, 12) 1, d 5 5 | Gas 2 | 
BEP Nenasu ee topo. | 10.-; 10.6 | 
se : 100 
1000 mers. of assimilated glucose, i.e. per 400 mgrs. carbon — 
gS ie teen a mers. N is fixed after that time, that is 6°/, N on 
the weight of mould. This value I will call nitrogennumber. 
After 4 days the accumulation was fairly the same. The nitrogen 
number was decreased to 5. Nearly all the ammoniumnitrogen (ca. 
13 mgrs.) was taken up by the organism, for with Nessiur’s test 
the liquid gave but an insignificant reaction. The remaining 7 mgrs. 
are furnished by the nitratenitrogen. 
1) An analysis of this water made in October last gave: 
solid substance 461,3 mgr. p. L. SO," 60,5 mgr. p. L. 
org. rs Tt eS rs CI OY es eee ere 
reduction power 3,5 mgr. O, p.L. (K MnO.) | NOs' traces 
dissolved Og 4,54 cM? p. 5. NO,' absent 
free CO, Py MSE 5: NE st 
fixed CO, 126,8 Sane Silieie acid (Si0,) 2,0 mgr. p. L. 
Total CO, 127,2 Beh gy ae Al, O3 + Fez Os oe ae aak en 
Temporary hardness (Preirer-WARTHA ): 8,07° | CaO Wie) A ees 
Total 8 ( : ):9,43° | Mg O EN 
Permanent = ( 7 ): 1,369 ‘Cu and Pb absent 
Mg A ( ): 2,02° | Na,gO 120,2 Bye 
Total . (CLARK) :9,29°; , from rest of combu- 
Shion: 19808 ees 
2) With this corresponds somewhat more than 26 mgrs. nitrogen. 
3) The temperature in these experiments was 25° CG. 
4) Addition of 5 mgrs. boric acid. 
