1050 
TABLE II. METABOLISM OF NITROGEN. 
50 cm3 tapwater, in which dissolved 2 pCt. glucose (free from water), 0,15 pCt. 
ammoniumnitrate, 0,1 pCt. potassiumchloride, 0,05 pCt. crystallised fosforic acid , 
0,1 pCt. crystallised magnesiumsulfate, 0,1 pCt. calciumnitrate (free from water). 
Temp. 34° C. 
| Days | Quantity of | ‚Reaction of the culture liquid 
; _after | nitrogen fixed | Growth and | with 
fation| omge | Per formation’) | nssorz, |Pipterylamin- 
| | 
| 3 19.3 igor hardly any spores slight | strong 
248. 4) 4 20.3: 20.7; 19.6 | =, , very few 2 = a 
5 5 17.5 A ep „ rather strong ij 
6 ie 11.8 Ee te » » ” 
ue | 9 9.9; 10.4 vty dathersmany’ „u a + 5 
JE 8.8 RE Ee en . 
(074.18 ol 10.6 tre A WAL mt " ” : 
11 19 10 | eos » » » » ” ” 
Excepting nr. 1 where only 80°/, of the glucose was used, all 
the glucose in the other culture tubes had been assimilated. 
The dry weight obtained from them I have not determined in 
these experiments. For this my earlier investigations may be compared *). 
Notwithstanding the quantity of fixed nitrogen had decreased on 
the 4t day from 6 to 5°/, of the assimilated carbon, the absolute 
quantity was not lower. On the contrary, a slight increase was 
observed caused by the glucose still present after 3 days. The mitro- 
gen excreted by the growing mould is assimilated again during the 
formation of new cells. When all the glucose had been used, which 
was already the case on the 4!" day, nitrogen only was excreted. 
The consequence was a considerable lessening of the quantity fixed 
in the organism. After 5 days (nr. 5) only 17.5 mgrs. remained 
fixed. This was accompanied by a decrease of the nitrogennumber 
as graphically demonstrated (fig. I). (see p. 1051). ; 
After 5 days it was already decreased to 4.4, after 7 days to 3, 
after 9 days to 2.5. Then it undergoes but insignificant changes. 
1) The use of very pure chemicals free from manganese causes the bad spore 
formation. 
2) Folia Microbiologica, Bd. 1 p. 422 and these Proceedings XV p. 759. 
