1054 
! 
oy Ao IIR oor ae Z 3 
= 2 
—- -§ © NY | WD - SS > 
© (0 KOA De SS Mgr N in the SE 
lS BS AED LD nt = En mould Gene > 
ei en Ls atten Silly genes FS ASG ees 
Do) RS _ bon a or a 4 oge) Q Q 3 °° 8 
ae Sue tke ee . . Nitrogennumber = *" ga S 
DOE RER ES a — OZ zE 
es i = Band a ; Ode T Zp os 
a Sh o M N a mn ian et 
RET ED AN ammonia fn Ae 
. . AD = 
HS Neo VO, ep ae Ne ; i oe eS ker) 
= 3 3 in solution. rè = = 2 
= 5 = os Gos 5 
SA ies et Oe Soa alone nj - = = ke) = Tm 
OE HV NA TEA pe ot EE Sum. SP Sn — 
=O GK Meo Do © : p © a © < 
Sage ee RE 
. . = 
Sen ey ee me Assimilated ~ abe = > 
ao » 2 jen & de = 
SS Cece. Rate ey faa Pe. | = = 
SS eS IP D © @ §!ucose = S) 
= > = oO = Et a > Z 
ay ye a eden é 
= =) =) = 
= = 5 
ey ei ek cere Recess co eb S 
Se Pe ries, Number of days 2 
a 5 é o 
Ss (3 sos = “s rs »  {alterinoculation| > 
< 
ui n 
= |- = —— u 
— — — — 2 > 5 by | 5 
Npe to MS AN am ci 3 a 
— 1 ONE fete SE mould 5 = 
: SS = SS 
rN IS on aD Me etn Eee Ar =) 
Sais Ze : . & Nitrogennumber ten = 
i | or _l ow (=>) Do JE it +-+U so 8e) a) Sa Zz 
=) = KS ON 5 
o le) OO Ee > = ro) 
= = Mgr. N. as = En sl 
SE a ON os : Risse Sees Qa 
DR PD i, a ammonia od eos ee m 
Coys 160 p_ 3 | © 4 on ; : OD If ae @ > Z 
=| | | in solution. ee 
=: =: eS) 2 
i 5 : Rete IE © = Seuss 
Sy ours D D> D iS. swe 
SS a = Gt ee | Sum he SN 
és) GA LE ej) Coe oo zine ome RRS 
= = = de Sn 
nd = = Assimilated 5 a4 
vS S&S Boo fo we IE 5 au fen 5 
SD eon NE glucose © ms 
= 3 = : pes a 
3 = = in pCt. RE 
= =: =: 
> Ee zi | = 
any losses of nitrogen, so, Aspergillus niger is able to reduce nitrate 
to ammonia. 
Furthermore it is proved that the nitrogennumber of a mature 
mould layer with glucose or levulose as exclusive organie food for 
Aspergillus niger, independent of the souree of nitrogen, amounts 
about to 2,0. 
Notwithstanding a young mould culture sometimes contains 2 to 
2'/, times as much nitrogen as a corresponding old one, such a 
young, duly washed layer, when boiled with distilled water gave 
no trace of the above named anorganie salts which had been added 
to the medium as exclusive source of nitrogen. Here thus, too, the 
