( ' ) 



J ()"''„ caiiesugar 

 inveHed 147„ 



lOVo caiiesugar 

 inverted 12.4''/„ 



lOVo eanesiigai' 

 inverted 19.17, 



87o canesngai' 

 inverted 16.6"' 



10"/,, eanesngar -f- ■^•57,, lacviilose 



12.57. — Vt '" 



1 + YT 



107u eanesugar -|- 57,, glucose 



= 12.4 



9.77o 



1 h 



1 -1 



^ 2 a 



12.4= 9.9 



10°/„ eanesugar -f 107o glucose 

 12.47„ \ 



87o eanesugar -\- IG7,, glucose 

 8.27„ 



19.1 =12.7 



1 + 



1 h 



16.6= 8.3 



It was now to be expected that many other neutral substances 

 would also retard the in\ersioii according to their capacity of 

 absorbing the enzyme radiation. 



These are some of the figures obtained: 



Under the same conditions the same enzyme-(piantities inverted of 



lOVo eanesugar 38.57„ 



lOVo eanesugar -f 57„ urea 28.5"; 



10"/o eanesugar -{- 5"/, mannitol 33.— "/„ 



107o eanesugar -]- 5"/, erythrite 28.— "/„ 



10"/„ eanesugar -f ^Vo glucose 29.8"/o 



In another series : 



10"/' canesuffar 



58.1V„ 



10"/„ eanesugar -f 5"/., dulcitol 50.4"/,, 

 10"/„ eanesugar + 5"/„ glucose 47.8"/, 



There seems to be some kind of relation between the asymmetric 

 carbon atoms and the absorption. 



In the case of inversion of more diluted solutions of eanesugar 

 the above-mentioned simple relations will no longer exist. If we 

 diminish the initial concentration, a dilution will soon be reached 

 where a part of the radiation does not reach a sugar molecule 

 in time, but is either finally absorbed by the water or when arriving 

 lias, in anv oase, become too \\'eakened to cause inversion. The 



