(8) 



quantity of canesugèxr inverted by a given amount of invertin will, 

 therefore, go on decrea-sing. In the end, however, we shall arrive 

 at an initial concentration where, within the sphere of action of an 

 enzyme particle, tAvo canesugar or in^•ert sugar molecules can no 

 longer shade each other. From this point, the inversion caused by 

 the given enzyme-quantity will be just proi)ortionate to the canesugar 

 concentration. Then, during the whole of the process, the reaction 

 velocity merely depends on the average number of canesugar mole- 

 cules present within the active radiation sphere of an enzyme particle. 

 The following are some of the figures obtained which always 

 exhibited the same regularity. 



Concentration 

 canesugar in grms. 

 per 100 cc. 

 0.05 

 0.1 

 0.125 

 0.25 

 0.5 

 1.— 

 2.— 



Another series gave 



Concentration of Inverted 



canesugar in gr. per 100 cc. in grms. per 100 cc. 



3 0.86 



4 0.95 



5 0.96 

 7 0.93 



The fact that, in \'ery dilute solutions, the enzyme action really 

 proceeds as a unimolecular reaction according to the formula 



k^z-log- Wcis further again confirmed by experimenting with a 



solution containing 0,096 7o of canesugar. 



Up to the present wq have for the sake of convenience disregarded 

 the synthetical action of the enzyme rays. T.ight, being a catalyzer, can 

 act eitlier as a synthetical or decomposing agent, so we must expect the 

 same from the enzyme rays. That \\q often do not notice such 

 action is due in the first place to the secondary change of the 



