( 85 ) 



Finally, probably by further division, the spore is entirely filled up 

 with a granular, strongly stainijig mass. 



In the meantime the wall of the spore has become more prominent. 

 The number of spores, as was said above, is not constant and some- 

 times one finds only a single spore in one cell ; in some cases however 

 about 16 are counted, which then are smaller than the normal ones. 

 When the spores are ripe, the free cell, as a unit, falls asunder 

 and the spores become free in the ascogonium, the contents of which 

 are further formed by the epiplasm of the free cells and the remainders 

 of the protoplasm which in the beginning did not take part in the 

 formation of free cells. 



The spores do not fill up the ascogonium entirely but form together 

 a peripheral layer. Between the spores one finds an intermediate 

 substance, which is strongly stained by orange-G. So Ikeno's opinion 

 that the polygonal appearance of the spores is aa optic illusion, is 

 correct ^) 



Monascus Barkeri. 



The first stages of the development of the perithecium of this 

 form agree with those of the first species dealt with. Here also I 

 have not been able to state an open communication between polli- 

 nodium and ascogonium. The latter, wiiich here lies more parellel 

 to the pollinodium, is also divided by a cell-wall into two cells, the 

 posterior of which forms the final ascogonium. Only after it has 

 become surrounded, at first rather loosely, by hyphae, it greatly 

 increases in size. As long as it is small, sections of the whole 

 perithecium — ascogonium with enveloping hyphae — show much 

 resemblance with figures 17, 18 and 25 by Barker"). Together with 

 the volume of the ascogonium the number of nuclei also increases. 

 The ascogonium is now rich in protoplasm with many vacuoles. 

 A first indication of a division of the protoplasm into parts — especi- 

 ally concerning the central part — is observed by the occasional 

 appearance of long-stretched vacuoles. The nuclei are here and there 

 seen in couples and also a few bigger nuclei are seen, which I 

 presume to have been formed by the fusion of two of the smaller ones. 



In a following stage free cells have formed, each with a single 

 pretty large nucleus. This stage strongly reminds us of the corre- 

 sponding one with M. purpureus. The proto[)lasm of the free cells 

 is in most cases much denser than in fuj-nier stages; in few cases 



1) Ikeno 1. c. pag. 267. 

 ') Barker 1. c. 



