( 86) 



only it is still foamy. It very often occurs that the free cells have 

 assembled on one side of the ascogonium. Besides this one linds then 

 in the ascogonium a fairly strongly developed lining layer of proto- 

 plasm and one or a few very large vacuoles. This condition reminds 

 us of figs. 22 and 29 of Barker. The protoplasm which has taken 

 no part in the formation of free cells contains very few or no nuclei, 

 so that it is probable that several nuclei soon degenerate. 



The single nucleus of a free cell now successively undergoes three 

 divisions, so that at last eight nuclei are present. The two nuclei 

 after the first division and the four after the second, do not always 

 divide at the same moment, so that also cells with 3, 5, 6 and 7 

 nuclei are found, but then in the first case e.g. one of the three 

 nuclei is bigger than the other two. 



When two divisional nuclei — here again consisting of a strongly 

 staining grain — have arrived at a certain distance from each other, 

 one often finds between them a more or less complete band of a 

 somewhat lighter colour than the nuclei themselves but darker than 

 the protoplasm of the free cell. These divisional figures agree pretty 

 well with Ikeno's representations for Taphriua Cerasi '). 



After the eight nuclei have formed, spores arise with the nuclei 

 as centres. These spores become free in the ascogonium and further 

 behave like those of Monascus purpureus. 



The manner in which in the two forms described, the nuclei for 

 the spores are developed, differs in important points, but agrees in 

 a remarkable degree with what has been described by Ikkno for 

 Taphrina Kusanoi and T. Cerasi "). 



Both forms agree in this respect that in the ascogonium free cells 

 are formed with originally two nuclei, which fuse into one, from 

 which single nucleus the nuclei of the spores arise. This has induced 

 me to see in the free cells in the ascogonium of the genus Monascus 

 the homologon of an ascus, especially on account of Dangeard's 

 investigations on the Ascomycetes '). 



With the remaining Ascomycetes, e.g. Pyronema confluens *) and 

 Ascobolus *) the fusing of the nuclei would, according to this repre- 

 sentation, only be shifted in time and place compared with Monascus 



1) Flora Band 92 p. 1 (fig. 24 and 27). 



2) Flora Band 92 p. 1 (fig. 24 and 27). 



3) Le Botaniste 4 Serie, pag. 21—58, 1894—1895. 

 i) Harper, Ann. of Bot. vol. XIV, 1900. 



"j Harper, Jahrb. f. wiss. Bot. Band XXIX, 189G. 



