( 108 ) 



The network-volume of the same white blood corpuscles was 

 further investigated with the aid of mixtures of serum and different 

 quantities of water. 



Volume of white corpuscles 



(1) in undilated serum 35.75 



(2) in serum mixed with 20 "/^ water 39 



3 „ , „ „ 40 „ „ 41.75 



^ ); n n n ^^' v n ^^-^ 



From (1 and 2) we obtain the equation : 



120 (35.75— j;) = 100 (39-jj) 

 p = 19.5 

 From (1) and (3) we obtain the equation : 



140 (35.75— jj) r= 100 (41.75— p) 

 p = 20.6 



From (1) and (4) we obtain the equation. 



150 (35.75— |>) = 100 (43.5— jj) 

 ^ = 20.3 



It will be seen, that the figures which are found for j^ agree 



11 -.1 1 .1 rpi • 19.5 + 20.6 + 20.3 „„„, 

 very well Avith each other. I he mean is =20.04, 



20 04 

 i. e. caicultated at 35.75 :rrr^^ X 100=56%, a number that cor- 

 responds well with that obtained by the NaCl solutions. 



The observation might be made, that the numbers 54.5 or 56 

 cannot give the exact volume of the protoplasmic network as by cen- 

 trifugalizing only the precipitate and not the real volume of blood- 

 corpuscles is determined. I have, however, convinced myself by 

 experiments, that this has no perceptible influence on the percentage 

 of the network substance. 



This may be clearly observed inter alia when by means of acce- 

 lerated velocity of the centrifugal machnie we compress the precipitate 

 a little more. 



The percentage of j) is hardly or not at all changed by it. It 

 must, moreover be borne in mind, (I) that for one experiment all 

 fluids are centrifugalized at the same time and (II) that the figures 

 of the last experiment, 39, 41, 75, and 43.5 are a little greater 

 than corresponds with the real volume of the cells, because there is 

 still fluid among the cells, the same is the case with the number 

 35.75 with respect to which the percentage has been calculated. 



A second observation may be made viz, that in the calculation we 



