( 372 ) 



The epithelium was invariahly obtained by carefully scraping it 

 from the organ of an animal just killed. The scrapings were distri- 

 buted in a little fresh blood-serum or in a Na Cl-solution of 0.9% 

 and afterwards filtered through a small filter of unprepared gauze, 

 in order to have the cells as far as possible quite free or in small 

 aggregates. Equal quantities (± V* c.c.) of the fluid thus obtained, 

 were measured off and inserted in test tubes in which were equal 

 quantities of the salt-solutions, to the influence of which the epithe- 

 lium cells were to be exposed. After exposure for half an hour 

 equal quantities of the mixtures were put into funnel-shaped tubes 

 to be subjected to the influence of centrifugal force. "When the 

 level of the sediment had become fixed, the volume of the epithe- 

 lium-column was considered to be determined. If after being centri- 

 fugalized for a short time, the small column of cells did not appear 

 to be quite homogeneous, by far the greater part of the clear fluid 

 was pipetted off', the small column with the remainder of the fluid 

 stirred by means of a platina wire into a homogeneous mixture and 

 again centril'ugalized. ]f this was necessary with one of the tubes, 

 the same was done with the other three. 



I. INTESTINE-EPITHELIUM. 



Experiment I. 



Epithelium of the small intestine of a horse, at about one meter's 

 distance from the pylorus. 



The epithelium is distributed in a little blood- serum of the same 

 animal. 



Salt-solutions employed | Volume of the epithelium 



It will he seen that the volume of epithelium regularly decreases 

 with the rise in the concentration of the salt-solution. 



') Of. Verslag der Yergad. Kou. Akademie, April 21 1897 and May 28, 1898. 



