1564 
Physiology. — “Further observations on the relation between the mus- 
cular tonus and the amount of kreatin in the muscles.” By 
Prof. Dr. C. A. Pexetuarine and Dr. C. J. C. van Hoocennvyze.” 
(Communicated in the meeting of March 25, 1916). 
Our previous inquiries showed us that the tonus of the muscles 
is attended with an increase of the quantity of kreatin. For further 
observation we obtained through the kindness of Prof. Manus a cat 
that had, for some time been deprived of the tonus of the muscles 
of the left foreleg. 
This had been done in the following way : 
On the 27% of April 1915. Mr. Srorm van LEEUWEN had performed 
an extradural transection of the posterior roots of the 5, 6, 7% 
and 8t cervical and the 1st, 2°¢ and 3 thoracic posterior roots. 
After the wound had been sewn the left foreleg appeared to be 
insensible; the animal could nevertheless move it. The wound healed 
soon. The leg remained insensible and limp, but capable of voluntary 
movements. In walking the toes were doubled up under the weight 
of the body so that the dorsal side of the carpus rested on the 
ground, which gradually caused a slight decubitus. In every other 
respeet the cat was in a normal condition. 
On the 15 of September the cat was killed by chloroform. 
Immediately the left and the right Mm. triceps and biceps brachii 
were excised, cut into pieces, and put in flasks of a known 
weight; then weighed and boiled for five hours with ten times their 
volume of 1°/, HCl, evaporation being prevented by means of a 
reflux condensor. The fluid in which the muscles had by this time 
been broken up altogether, was then boiled under neutralization. 
After being cooled the volume was measured and filtered. Of this 
filtrate a measured portion was evaporated down to a definite 
volume. 
10 ee. of this fluid together with 20 ee. normal HCI was heated 
to and maintained at 115° C. for half an hour in the autoclave in 
order to effect a complete transformation of the kreatin into kreatinin. 
Subsequently the content of kreatinin was determined after Forty. 
The result was: 
Icey: 
Right 13.50 grm. Left 1517 grm. 
Onl Nn 1.953 mgrm. of kreatinin per J grm. muscle 
Barcie pis: 
Right 49 grm. Left 48 _grm. 
2.310 ,, 2.140 merm. of kreatinin per 1 gem, muscle. 
