1567 
The temperature of the room was now maintained at about 15° C. 
night and day. One cat was walking freely about the room; the 
other animals were locked up in the hutches for some time. When 
„a cat was killed an estimation was made in the usual way of the 
amount of kreatin (expressed as an amount of kreatinin) in the right 
and the left triceps brachii. 
The results were the following : 
IV. moving about 10 days, Left 3.548, Right 3.282 mer. 
V. locked up 1 week, eel) eer OON oe 
VI. - » 2 weeks, eo oS. eee 22902) 
VII. 7 EET oO eee EO 
VIII. 5 eee oy 2800, 2.040) %, 
It being obvious, then, that the enforced rest, at least after the 
lapse of a fortnight resulted in a fall of the amount of kreatin, one 
of the cats of which the amount of kreatin might be supposed to 
have been reduced in consequence of its having been in the hutch 
for two weeks, was set free, in order to see whether unrestrained 
movement would raise the amount to its normal degree. When the 
animal had been walking about for nine days in the room maintained 
at 15° C., it was killed. An investigation of the left and the right 
triceps brachii yielded the following results: 
IX. Left 3.620, Right 3.680 mer. 
Three more cats were examined. They had been living in the 
hutehes, while the temperature of the room had been maintained 
at += 23°C. The health of the animals was normal also at this 
temperature. In each case the body-weight had augmented. 
We found: 
X. locked up 2 weeks, Left 2.900, Right 2.990 mer. 
XL ; 3 en 26108” Ut OIO LTE 
yy em dan iy 2.530 eee 20 w 
from which we see that the content of kreatin, obtained from the 
muscles of cats that had been living in a hutch for some time, at 
a constant temperature, was on an average: (Left): 2.879, (Right): 
2.836 mgr. kreatinin, while the muscles of the cats that had been 
walking about, or had been locked up in a narrow space at a low 
temperature of the outside air yielded the averages: Left: 3.520 
and Right: 3.506 mgr. of kreatinin per gram muscle. 
These means, derived from 5 cases (I, Hl, HI, IV and IX) differ 
very little indeed from the average 3.397, which we found previ- 
co 
ously in 5 other cases. 
