SPOROZOA OF THE FISHES OF THE ST. ANDREW'S REGION 

 SESSIONAL PAPER No. 39b 



33 



adhering to the free surface of the myxosporidium (PL IV, Figs. 7 and 8) i.e. the 

 surface not in contact with the epithelium. In fresh preparations the appearance 

 is that of budding from a parent organism (PI. IV, Figs 7-8). For a time this 

 was thought possibly to be the case for some of the adherent individuals. An 

 examination of sections has shown a sharp division between the myxosporidium 

 and C. acadiensis. No other spores than those of C. acadiensis were found in the 

 gall bladder of U. chuss. 



5. Goussia gadi Fiebiger. 



The haddock in which this parasite was found was caught on the silth of 

 August. The abdominal organs were cut out and the fish was put on ice. Next 

 day when the fish was being prepared for the table it was proclaimed unfit for 

 cooking on account of a creamy exudation in the dorsal part of the body cavity. 

 It was at this time that the fish was brought to the notice of the wi'iter. On ex- 

 amination a creamy mass, yellowish white in color was found adherent to the inner 

 surface of the air bladder. This had the appearance of being due to the breaking 

 down of the lining membrane. The kidneys and surrounding muscular tissue 

 appeared quite normal. A microscopic examination revealed the presence of numer- 

 ous ellipsoidal spores arranged in groups of four in the creamy mass. "Wet" 

 smears were fixed in Schandinn's sublimate-alcohol mixture and in Hermann's 

 platinic chloride-osmium-acetic mixture. They were subsequently stained in 

 Grenacher's borax carmin and in Delafield's haematoxylin. The preservation 

 proved to be not all that could have been desired but seems sufficient to determine 

 the systematic position of the parasite. 



The macerated condition of the cells of the air bladder both when examined 

 fresh and in preserved preparations has made it impossible to determine any of 

 the schizogonic or syngamic stages. There can however be no doubt that the form 

 is tetrasporous from the almost constant occurrence of the spores in groups of four 

 usually surrounded by a structure which appears membranous in the preparations . 



a b c 



Fig. 4. Goussia gadi Fiebiger. a, spore stained with Delafield's haematoxylin 

 showing the two sporozoites with their nuclei, X 1900. b, tetrad of spores inclosed 

 in mass which is probably remains of host cell ; drawn from fresh preparation, X 970. 

 c, two valves of spore cell drawn from preparation fixed in Hermann's fluid, X 1900. 



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