52 MARINE AND FISHERIES 



5 GEORGE v., A. 1915 



tissue found to contain it, might throw fresh light upon the problem of its presence 

 in the thyroid gland. 



Further, should kelp be utilized extensively as a source of potash for 

 fertilizing purposes, as seems not unlikely from recent investigations,* the iodine 

 present in the kelp would become the chief bye-product of such an industry; hence 

 additional information as to its distribution and variation in different algae seemed 

 also likely to lead to results of value. 



With the permission of the Biological Board of Canada, I collected material 

 during August and part of September, 1913, at and near the Biological Station at 

 Departure Bay, B.C. This material I have subsequently analysed in the Physio- 

 logical Chemical Laboratory of the University of Manitoba. 



A large number of specimens of different species of algae were obtained, and 

 also specimens of representatives of most of the animal phyla. The selection of 

 the latter was made more or less at random, and analysis of different tissues of the 

 species examined was also not systematic; the investigation is to be regarded as 

 preliminary, with the purpose of indicating the direction for further work. Com- 

 plete examination of the tissues of the dog-fish Sqiuilus sucklii was carried out. 



The various specimens were collected at the following points : At the Biological 

 Station, or at points within half a mile of it (including Jesse Island) ; north-west of 

 the Station, in the neighbourhood of Hammond Bay and the Lagoon; near Snake 

 Island, two miles east of the Station; near Protection Island, two mUes south-east; 

 in False Narrows, about eight miles south-east of the Station; north of Breakwater 

 Island, two miles east of False Narrows; on Mudge Island, two mUes south of 

 False Narrows; 



Methods of Preservation and Analysis of Material: The algae were air-dried, 

 further* dried over sulphuric acid, and finally heated at 100° C. to constant weight. 

 The remaiaing material (except in the case of a few shells and tests which were 

 air-dried) was preserved in absolute alcohol, or in a few cases in dilute formaldehyde. 

 In all cases before analysis the alcohol (or formaldehyde) was evaporated and the 

 material heated to constant weight in the water oven at 100°, so that the results 

 are all expressed for dry tissue. 



Hunter's method of analysis has been adopted.' It has been shown by 

 Seidell* and others that this is a very accurate method for analysis of small quan- 

 tities of organically combined iodine. The results given by it are slightly higher 

 than those obtained by the Baumann method or its various modifications, one or 

 other of which have hitherto usually been employed. 



» See "Fertilizer Resoiirces of the United States," Senate Document, No. 190, 62nd Congress 

 2nd Session, 1912. 



» Hunter, J. Biol. Chem., 1910, 7, 321. 

 «Cp. SeideU, ibid., 1911, 10, 95. 



