23 



evitámos que as larvas viessem 

 á abertura do frasco. 

 21 - Renovámos a agua. Todas vivas. 

 22 h. examinando o frasco, encon- 

 trámos, por baixo da rolha 

 de vidro, uma pequena bolha de 

 ar. Retirámos a bolha. As 4 lar- 

 vas foram transportadas para um 

 bocal de vidro ; a de Culex mor- 

 reu alguns dias depois, as de 

 Stegomyia deram ninfas a 23, as 

 quais deram imajens a 25. 



II. Novembro. 



8 larvas de Stegomyia (4 sem foliólos 

 e 4 com foliólos) e 2 ninfas, 



Vidro fechado ás 12 h. 52 



15 h. Mortas as 2 ninfas. 



17 h. Morta uma das larvas sem foliólo. 

 Dia seguinte : 7 h. pareciam todas mor- 

 tas; abrimos os vidros; trans- 

 portámos todas as larvas para 

 um bocal aberto com agua: de- 

 ram logo sinais de vida. 



12 h. moviam-se todas perfeitamente. 

 Dias depois algiímas morre- 

 ram. Ficaram apenas 3 que 

 deram ninfas a 23., as quais de- 

 ram imajens a 25. 



III. 23 de Novembro. Agua recentemen- 



te fervida. 1 larva de Culex jati- 

 gans, 1 de Stegomyia e 1 ninfa 

 de Stegomyia. 

 Vidro fachado ás 7,50. 

 Ninfa morta ás 9,30. 

 Larva de Culex morta ás 10,30. 

 « « Stegomyia & « 12,15. 



IV. 26 de Novembro. 



2 larvas de Stegomyia de tamanho 

 medio e 2 de Liniatus do mes- 

 mo tamanho. 



Vidro fechado ás 20 h. 15. 



27 - 12 h. renovada a agua. Todas vivas. 



28-22 h. Todas vivas. 



29 -Examinando pela manhã o vidro, 

 vimos uma bolha de ar. Larvas 

 ainda todas vivas. 



V. 20 de Dezembro. 

 Agua de chuva arejada. 



larvae coming to the mouth of 

 the jar. 



21st. Water renewed. All larvae alive. 



10 p. m. Saw a little air bubble un- 

 der the glass cover; removed 

 the stopper and transfered the 

 4 larvae to an open jar. The 

 Culex larva died a few days 

 afterwards the Stegomyia larvae 

 pupated at 23rd. and the ima- 

 gines hatched at 25th. 



II. November. 



8 larvae of Stegomyia (4 without and 

 4 with leaflets) and 2 pupae. 



Jar closed at 12,52 p. m. 



3 p. m. 2 pupae dead. 

 5 p. m. 1 larva without leaflets dead. 



Next day: 7 a. m. all the larvae looked 

 dead. Opened the jar and tran- 

 sferred the larvae to open vessel 

 with water and found thai all 

 the larvae were still alive. 



12 m. All the larvae show movements. 

 A few days afterwards some 

 larvae died. Remained only 2 

 larvae that pupated at 23rd; the 

 imagines hatched at 25th. 



III. November 23rd. 

 Recently boiled water. 



1 larva of Culex fatigans, 1 of Ste- 



gomyia and 1 \i\xpd.oi Stegomyia. 

 Jar closed at 7,50. 

 Pupa died at 9,30. 

 Larva of Culex died at 10,30 a. m. 

 Larva of Stegomyia died at 12,15 p. m. 



IV. November 26th. 



2 larvae of Stegomyia half grown and 



2 larvae of Limatus of the same 

 size. 



Jar closed at 8,15' p. m. 



27th. 12 m. Water renewed. All lar- 

 vae alive. 



2Sth. 10 p. m. All alive. 



29th. Saw a little air bubble, having 

 appeared into the jar during the 

 night. Larvae alive. 



V. December 20th. 

 Aired rain water. 



