70 AGRICULTURAL EXPERIMENT STATION. 



severe cases of the aestivo-aiitumiial type were traceable to 

 Italians engaged in railroad work, several of whom had had the 

 disease, but were then apparently well. 



The case against Anopheles is complete, so far as its relation 

 to malaria is concerned, yet the work of the physician must be to 

 cure the individual if he can, for if every case of malaria were 

 absolutely cured before winter set in not all the Anopheles in the 

 localitv could start it again next vear ! 



CHAPTER VII. 

 MOSQUITO STUDY AND ARRANGEMENT. 



HOW^ TO COLIvPXT AND PRESERVE MOSQUITOES. 



A collection of local mosquitoes in larval, pupal and adult 

 stages should be part of the outfit of every health inspector in 

 the State, and such a collection is easily made. To obtain all 

 stages of the common house mosquitoes, Culex pipiens and C. 

 resiuans, it is only necessary to place out-doors a wooden pail 

 or tub half filled witli water, with a little vegetable mold or dead 

 grass to start organic life. In a day or two egg-boats will be 

 found on the surface of the water and, perhaps, eggs of Ano- 

 pheles as well. These species lay their eggs at night and the 

 boat ripens during the day following, young appearing in about 

 thirty hours from the time of oviposition. If it is desired to pre- 

 serve the egg boats, they can be placed in a little tube or bottle in 

 95 per cent, alcohol. If this is tighth- corked the specimens will 

 keep for a long time without attention. If the egg-boats are left 

 in the pails to hatch, the developing larvae may be watched from 

 day to day until the first pupa appears. Then, whatever larvae 

 are needed for preservation may Idc taken and put into 75 per 

 cent, alcohol. In a few days the 75 per cent, alcohol should be 

 poured off and 95 per cent, alcohol added for permanent pres- 

 ervation. Tightly corked and alcohol added from time to time, 

 as needed, these specimens will keep for years. As fast as pupae 

 appear they should be taken up with a wide-mouthed medicine 

 dropper and placed either in alcohol to be preserved like the 

 larvae or in a quart jar, covered with a gauze hood. In from 

 twenty-four to forty-eight hours adults will begin to issue and 



