134 AGRICULTURAL EXPERIMENT STATION. 



many more adults had emerged in all the jars, only a few larvse 

 were active in Jars 1-3, while all the larv?e were dead in Jar 4. 

 After seventy-two hours more adults had emerged, all the larvse 

 had either pupated or were dead and the experiment was closed. 

 A number of larvse were in apparently good condition in a check 

 jar when the experiment ended. The water in the experiment 

 jars cleared soon after the copper sulphate was added, while that 

 in the check jar. retained its cloudiness. 



Experiment 6: with larv;e and pupae of Citle.v pipicns breeding 

 in pails at New Brunswick. They were placed, together with a 

 small quantity of the breeding water ancl a little of the bottom 

 food material in four jars of city water to which the copper sul- 

 phate was added as follows : 



Jar I — I part of copper sulphate in 53552 of water. 



Jar 2 — I " " " " " 26776 " " 



Jar 3—1 " " " " " 13388 ." 



Jar 4 — I " " " " " 6694 " " 



Twenty-four hoiu's after the copper sulphate was added a few- 

 adults had emerged in all the jars, and the larvse were in appar- 

 ently good condition except a very few in Jar 4. After seventy- 

 two hours a few more adults had emerged in all the jars and in 

 Jar I the conditions were unchanged. y\ll the small larv?e and 

 a few of the larg-er larv?e were dead in Jars 2-4 and the remaining 

 larv.-e in these jars did not seem so active. After ninety-six hours 

 conditions were unchanged and the experiment was ended. 



Experiment j: with larvae and pup?e of Ciilex pipiens breeding 

 in a pool in a lot at New Brunswick. They were placed, together 

 with a part of the breeding water, in eight jars of city water and 

 copper sulphate was added to give the following proportions : 



Sixteen hours after the addition of the copper sulphate a few 

 adults had emerged in all the iars ; there were a few dead larvae 

 in Jars 1-3, many were dead in Jar 4, most of them were dead 

 in Jars 5 and 6, and all were dead in Jars 7 and 8. After forty- 

 eight hours more adults had developed ; there were a number of 

 active larVcT in Jars i and 2, some in Jar 3, a few in Jar 4, a very 

 few in Jar 5 and none in Jars 6 and 8. After seventy-two hours 



