64 THE MICHOSCOPE. Apr., 



Strong liquor Ammonia h drachm. 



Glycerine 2 ounces. 



Distilled water 2 ounces. 



Alcohol i ounce. 



Cell Culture of Fungi. — Most kind of fungi especially the 

 blue mold found on bread and on top of preserves may be 

 cultivated in cells and conveniently examined in these cells 

 with the microscope. The following is copied from Bessye's 

 Botany, page 240. 



The most accurate and satisfactory, but at the same time most 

 difficult cultures, are cell cultures. They are made as follows : — 

 glass, tin, or India rubber rings four to five millimetres high are 

 fastened to ordinary glass slides ; a very little water is placed in 

 the bottom of the cell so formed, to keep the air in it always 

 moist ; a small drop of the nutrient liquid, free from spores of 

 any kind, is placed in the middle of a cover glass of the proper 

 dimension, and in this a single spore of some particular mould 

 is placed ; the cover g^ass is now inverted over the cell. The 

 preparation must be placed in a warm and saturated atmosphere. 

 An ordinary bell-jar set over a plate of water, or better still, of 

 wet sand will furnish a very good moist chamber. 



Nutrient fluids are as follows : — First, boiled and filtered 

 orange-juice; second, a decoction of horse-droppings boiled and 

 filtered ; third, a saline solution as follows:— 



Calcium, nitrate 4 parts. 



Potassium phosphate. 1 part. 



Magnesium sulphate 1 part- 



Potassium nitrate. 1 part. 



Distilled water. 700 parts. 



Sugar ■ 7 parts. 



In some cases the sugar may be omitted. 



SPECIAL NOTICE. 



Radiolaria material. — Those who are sufficiently interested 

 in Rev. F. B. Carter's article on pages 49-55, to wish to study 

 the subject can obtain some material by addressing him at 

 Montclair, N. J., and enclosing stamps. 



