THE BUD-ROT Ot PALMS IN INDIA. 26l 



The trees were two palmyras and a coconut about 8 feet high, 

 and the inocidations were made as before. After 8 days the inocu- 

 lated sheaths of two of the trees were examined. In the coconut one 

 large brown spot, about i inch in diameter, and two lightly coloured 

 smaller spots were found on the inoculated area. In one of the pal- 

 myras there was a large irregular slightly discoloured blotch. The 

 wrappings were again replaced and not removed until a month from 

 the time of inoculation. Little progress liad occurred in the 

 coconut palm, the large spot being now much sunken but scarcely 

 larger than when seen before and it had not penetrated the 

 sheath. In the palmyras the inoculated sheaths had been penetrated 

 and those next below infected but not completely penetrated. 



V. The material used was a drop of water containing zoospores 

 from a slide on which sporangia had discharged. 



The inoculation was made by opening the folds of a ^'ery young 

 leaf in the apical shoot of a young palmyra, pouring in the drop, 

 closing the folds and securing them by a thread. 



After 14 days the inoculated leaf was cut and examined, and 

 brown patclies were found extending through about 10 of the folds. 

 These were sectioned and found to contain liyphse of Pythium 

 palmivorum. i 



VI. The material was a small mass of mycelium from the 

 superficial web of Pythium palmivorum from a diseased palm. 



The inoculations were made as in the last experiment, on the 

 still folded leaf in the bud of two young palmyras. 



The inoculated leaves were cut and examined after 28 and .30 

 days respectively. Brown spots extended from the inoculated 

 place through three or four folds, and on sectioning hyph?D were 

 found passing through the stomata. 



The above cases show that the disease can Ije produced by arti- 

 ficial inoculation. In the first experiment the number of sheaths 



