1897 THE MICROSCOPE. 45 
QUESTIONS ANSWERED. 
Note.—Dyr. S. G. Shanks, of Albany, N V., kindly consents to recetve al. sorts of ques- 
tions relating to microscopy, whether asked by professionals or amateurs. Persons of ai 
€ ades of experience, fron the beginner upward, are welcome to the benefits of this depar* 
ment. The questions are nunibered for future » eference. 
247. Q.—Pleasegive the best method of preparing, stain- 
ing and mounting spermatozoa ~and oblige.—Investigator. 
A. Smear a cover glass with the spermatic fluid, let it 
dry and stain with eosine. Oruse Unger’s fluid: Methyl 
green, 10 grains; water, 1 0z; Hydrochloric acid, 1 drop: 
mix. This method willstain thespermatozoainthreeshades 
of green. 
Ehrlich—Biondi fluid (blood-stain) will stain spermatozoa 
beautifully. Let the prepared cover remain inthe stain 
for 24 hours. After staining inany of the above solutions, 
wash quickly in water and dry. Mount in xylol balsam. 
248. @Q.—Price of Practical Hints, etc., onthe Micros- 
cope by John Phin. General description, pages, etc., date 
of last edition.—R. H. D. 
A. Last edition, 1890, price $1.00, postage 8 cents, cloth 
231, pp. The book isintended for beginners. It contains 
descriptions of microscopical apparatus and accessories 
with directions for their use, also some general hints on 
collecting and mounting objects, with formule for mount- 
ing media and cements. It is practicalas far as it goes 
into the subject. 
SO LENCE =60S,S:1.P; 
Rapid Filtration.._C. W. Shackett in the Druggist’s 
Circular gives a description of a contrivance to aid in fil- 
tration which he devised and which he finds very useful 
in practice. The usual metal filter racks proving unsatis- 
factory to him in many cases, he procured some glass stir- 
ing rods about 3-16 of an inch in diameter, and from5 to 8 
inches long, one end being bent to form a small hook. 
