236 



The results, though still various and in some respects conflicting, are interest- 

 ing and instructive. They show certainly the different degree of susceptibility 

 in diff'erent breeds, and the loss or destruction of the vitality of the vaccine virus 

 with age. The wide diff"erence in severity and fotality, noticed in the two series 

 of experiments, is readily suggested by the fact that matter from two to nine 

 months old was principally used at Boudarewka. 



At this place in 1860 fifty-eight cattle were vaccinated, nine were very sick, 

 and three died. In most of these cases the matter was from five to nine months 

 old. Re-vaccination of thirty-seven with fresh matter resulted in the sickness 

 of five, of which three died. 



In 1S61 two hundred and fifty-seven were vaccinated inside of the institution, 

 and two hundred and twenty outside. Of the former, only five were very sick, 

 one hundred and seventy-seven were slightly ill, forty-two had some symptoms 

 of the disease, and but one died. About half of those outside had the disease 

 in a mild form. In one experiment two animals were vaccinated with slight 

 effect, and afterwards took the disease naturally, notwithstanding vaccination, 

 and both died. 



At the arrival of the commissioners in 1863 two hundred and ninety-five had 

 already been experimented upon by the Veterinary Surgeon Sergeeff, of which 

 fifty-one had slight symptoms, one hundred and thirty-six had some cough and 

 epiphora, thirty-three remained well, and seventy-five were not observed by 

 the inoculator. 



In three years Sergeeff vaccinated 1,028 animals, but used old vaccine matter, 

 except in forty -five cases, of which seventeen took very sick and four died. 

 The loss of contagious power in old matter is shown, further, by experiments of 

 the commissioners upon twenty-one of Sergeeff's subjects with fresh matter, of 

 which nine took sick, and four died. In another case, sixty-five head were 

 tried with fresh vaccine matter, and thirty-nine were severely aff'ected, and nine- 

 teen died, an unusual degree of fatality at Bondarewka. Matter from one to 

 nine months old was tried vipou foiarteen animals, all escaping infection ; but 

 upon re-vaccination with fresh matter ten became sick and three died. 



In one experiment two sheep were infected, and matter taken from them 

 was used successfully in infecting six cattle ; all were sick, and all but one died. 

 Vaccinated animals were frequently exposed to contagion with impunity, un- 

 less in cases in which the symptoms were comparatively mild. 



In 1860, the whole number vaccinated at Salmysche Avas 64; 36 took sick 

 after the first vaccination and 13 died; the other 28 were again vaccinated, of 

 which 16 sickened and 7 died. Of the other 12, 10 were a third time vaccinated, 

 and 4 a fourth time, with only one animal in each case slightly affected. Of 

 the Avhole number, 64, 53 animals were infected, and 20 died. No milder efffect 

 was produced by vaccine matter " of the fifth generation." Of three animals 

 vaccinated with matter of the second generation, two were infected and one 

 died, while four animals died out of seven infected with matter of the fifth gen- 

 eration. In most of these experiments, matter over 25 days old had no effect. 

 In 1851 there were 151 vaccinated, of which 69 remained well, 39 had slight 

 symptoms of disease, 43 had it severely, and 24 of them died. These 69 and 

 4 others slightly affected were re-vaccinated; 43 of which sickened and 17 died. 

 Of 27 vaccinated the third and fijurth time, 14 took sick and 8 died. In these 

 experiments some that were slightly sick at the first vaccination died as the re- 

 sult of the second. 



Some died after the third vaccination. Matter more than 9 days old was 

 found inefficient. 



In 1862, the third year, there were 51 deaths from 130 infections out of 167 

 vaccinations. It is a noticeable fact that of two breeds, the Baschkir and the 

 Kirgis, less than a fourth of the latter became slightly sick, while about two- 



