32 The Preparation of the Brain, &c. 



suflSciently stained a portion of it may be transferred to a slide, and 

 teazed out with needles in acid glycerine (that is, glycerine, 1 ounce, 

 hydrochloric acid, 2 drops). A cover glass is then put on, and 

 pressure exerted. This may be very easily obtained by means of a 

 strong spring clip. It is well not to mount the preparation per- 

 manently for a few days. By this method you get very good 

 preparations of the cells of the grey matter. (Dr. Beale.) 



The cord. If the cord is fresh it is quite stiff enough to allow 

 of sections being cut without a machine. They are placed for half 

 an hour in a J per cent, solution of bichromate of potash. Stained 

 with carmine, and mounted in dammar, in the same manner as 

 the sections of hardened cord. 



Dr. Kutherford finds the method of freezing of great service in 

 the examination of the fresh brain and spinal cord. The sections 

 cut from the frozen tissue are coloured in the dilute carmine, and 

 then teazed or mounted in glycerine I ounce, hydrochloric acid 

 2 minims, or glycerine 1 ounce, glacial acetic acid 5 minims. 



