Bacteria in Organic Infusions. 123 



Series C. Nov. 28th. Experiments tvith Turnip- and-Cheese 

 Infusion. — The infusion similar in all respects to that in Series B, 

 but prepared with a somewhat larger proportion of turnips ; therefore 

 of higher specific gravity, which was not numerically determined. 



Tubes 20, 21, 22, 23, Boiled and sealed approximately during 

 ebullition. Not subsequently submerged. 



Tubes 24, 25, Boiled and sealed approximately during ebulli- 

 tion. Subsequently submerged in boiling water during thirty 

 minutes. 



The tubes were preserved in the aii--bath as in Series A and B. 



Series D, Nov, 30th, — An infusion prepared as in Series B 

 and C, but brought to a sp, gr. 1031 by evaporation after filtration. 



Tubes 26, 27, 28, 29, Sealed cold. Subsequently submerged 

 in boiling water for thirty minutes. 



Tubes 30, 31, Boiled and sealed approximately during ebulli- 

 tion. Not subsequently immersed. 



Tubes 32, 33, Boiled and sealed approximately during ebulli- 

 tion. Subsequently submerged in boiling water for thirty minutes. 



Appearances in the Infusions, Series C and D, at the time of 

 Sealing and Suhmerging. — The appearances in the freshly-prepared 

 infusion were similar to those described above as characterizing 

 such infusions. 



Subsequent naked-eye examination of the tubes did not reveal 

 the slightest change ; they remained limpid. Specimens from each 

 group were opened and examined with the microscope after four 

 days, and the microscopic characters found to be unchanged : the 

 liquid was perfectly sweet. The remaining tubes were examined at 

 intervals before the end of December, being maintained during the 

 whole time at a temperature of 35*^ to 40^ C. in the air-bath ; they 

 equally proved to have remained unchanged when opened and 

 examined with the microscope, and were also free from unpleasant 

 smell. 



Series E. Nov. 28th. — Six porcelain capsules were heated to 

 redness, and nearly filled with the turnip-infusion used in Series C. 

 They were j^laceil on the air-bath under a glass shade. 



Capsules 1, 2. The infusion was unboiled. 



Capsule 3. The infusion was boiled in the capsule. 



Capsule 4. The infusion was introduced after it had been boiled 

 for five minutes in a superheated test-tube. 



Capsules 5 and 6. The infusion was that used in Capsule 4, 

 but a drop of distilled water was added to each of these tAvo 

 capsules. 



After four days the infusion in capsules 1, 2, 5, and 6 was found 

 to be teeming with Bacterium termo and Bacterian filaments. 



Capsule 3 was found to be cracked, and hence was discarded (it 

 swarmed with Bacteria). 



