PROGRESS OF MICROSCOPICAL SCIENCE. 
21 
They stain deeply in gold preparations, and aro then always 
puckered transversely. 
In gold preparations of the inflamed frog’s tongue, isolated primary 
bundles, identical in appearance and breadth with those of the inflamed 
cornea, are to be found. 
The depth of staining by gold shows that the constituent elements 
of the primary bundles undergo a chemical change in inflammation. 
The author has studied, by means of chloride of gold, the effects of 
inflammation in the quadrangular and in the long flat cells which 
cover the bundles in the interior of the cornea, but chiefly in frog 
corner sealed up in blood-serum, the latter method being found more 
certain to give available preparations. 
The only appearance observed, anterior to a complete destruction 
of the cell, was a division of the nucleus into two or more parts. In 
serum preparations the products of the division assumed the form of 
circles of highly refractive particles. Similar particles were sparsely 
scattered in the substance of the cell. 
The area of any one circular product of this division was always 
much smaller than that of the undivided nucleus. 
In regard to the stellate cells, the author questions the correctness 
of the accepted theory, which implies an identity of the cell and its 
processes with the visible protoplasm. He considers that the refractive 
particles, which constitute what is visible in the cellular protoplasm, 
are suspended in a fluid, similarly to the pigment-granules in the 
pigment-cells as described by Mr. Lister. The phenomenon described 
by German investigators as “ Zusammenballen” of the cell-processes, 
he attributes to a collection of the protoplasmic particles in the centre 
of the cell, similar to that which takes place in concentration of pigment. 
This opinion is borne out by a comparison of gold and osmic acid 
preparations. In conditions in which, by the former process, an 
isolated globular body is seen, osmic acid preparations show that the 
anastomosis of the thread-like processes remains complete. Reasoning 
analogically from the results obtained by gold in other tissues, he 
infers that it is what may be described as the contents of the cell and 
processes which stain by that method. 
Treatment by osmic acid is the only reliable method by which he 
has obtained satisfactory preparations showing the stellate cells in the 
inflamed cornea. The advantages of this mode of treatment are much 
enhanced by subsequent staining with red aniline, which especially 
differentiates the protoplasm and processes. Subsequent staining by 
hfematoxylin renders the nuclei visible. 
The only change, except that of destructive disintegration, observed 
by the author as a consequence of inflammation in the stellate cells, 
consists in the anastomosing processes being, in gold preparations, 
occasionally represented by fine darkly-stained lines, on which are a 
series of small globular swellings placed at short regular intervals, 
giving any one process an appearance identical with that presented by 
an ultimate nerve-fib rilla in a gold preparation. The same appear- 
ance is also to be seen in osmic acid preparations, and is suggestive 
of points of communication between the lumen of the process and the 
