PROGRESS OF MICROSCOPICAL SCIENCE. 
23 
most certainty in osmic acid preparations. They are found in groups 
in the wider spaces, in rows in the nerve-channels and between the 
primary bundles (corneal tubes of Bowman), and in large numbers in 
the tracts between the larger bundles. They are mostly round, some- 
times club-shaped, never pointed at two extremities as an elongated 
shuttle-shaped mass (that is, never spindelformig, spiessartig). A small 
minority consist of a double body formed by two rounded globular 
masses joined by a smooth isthmus. When stained by hfematoxylin, 
nuclei are found in either end, but not in the isthmus. The author 
infers that we have here a corpuscle in process of division. 
In rabbit corneas, in which inflammation has lasted about a week, 
some white corpuscles are seen with uneven contour ; and bulging 
outwards from, or lying close beside, them are bodies evidently nuclear, 
and which are affected by osmic acid and subsequent staining with red 
aniline, in a manner identical with the red blood-corpuscles seen in 
blood-vessels in the same preparation. The identity of the escaped 
nuclei with red blood-corpuscles is shown by a comparison of their 
respective size, evenness, colour, and contour. 
The author infers a production of red blood-corpuscles in inflamma- 
tion from the nuclei of the white blood-cells. 
In observations on human blood, and that of the mouse, by staining 
with hfematoxylin, he has found that while the great majority of the 
red corpuscles do not quickly stain in a weak solution, there are some 
which at once stain a deep blue, and that there are white corpuscles 
in which a narrow protoplasmic margin encloses a deep blue nucleus 
similar in contour and size to the stained red corpuscles. Amongst 
the red corpuscles of tho frog are a minority which are recognized as 
being red corpuscles by their size, smooth contour, and absence of 
granulation, but in which there is no hfemoglobin, and the nucleus 
quickly stains blue in solution of hfematoxylin, like that of the white 
cells. 
Transitions occur in which a less and less capacity of staining on 
the part of the nucleus takes place, pari passu, with an increase in the 
colour characteristic of hfemoglobin in the body of the cell. In the 
fully developed red corpuscle, the nucleus stains only after it has been 
for some time in contact with a weak solution of hfematoxylin. 
The author has observed in the blood of the mouse foetus the nuclei 
of the nucleated red blood-cell escape from tho larger cell, and then 
become indistinguishable in form and appearance from the small red 
corpuscles of the mature animal present in the blood under exami- 
nation. 
These observations, taken in connection with the bodies that are 
formed in the spindle-cells and white corpuscles in inflammation, sup- 
port, as the author believes, the doctrine of Wharton Jones, in regard 
to the formation of the red blood-corpuscles. 
The mode of formation of capillary blood-vessels he believes to be 
identical in inflamed and in fa3tal tissue. In studying this subject he 
has found special advantages from the use of osmic acid, with or without 
subsequent staining in hfematoxylin. The stages in this formation 
are as follows. 
