The Microscope. 85 



proceed at once to those forms, the Insects, for which this series 

 was planned and for which the preceding has been merely ex- 

 planatory. But before doing so we must explain what the seg- 

 mentation of the body, so prominent in the higher worms, the 

 arthropods and the vertebrates, means, and how it arose, and 

 this will be the subject of the next paper of this series. 



The best single work in embryology is '• A Treatise on 

 Comparative Embryology," by F. M. Balfour, published in two 

 volumes by Macmillan & Co. Next in order is " Outlines of 

 Comparative Embryology," by A. S. Packard, published by 

 Henry Holt & Co. On the points so far discussed in this series 

 the reader will find some hints in a short series of papers (un- 

 fortunately never completed) by Dr. C. S. Minot, in the Amer- 

 ican Naturalist for 1881. The phenomena of maturition and 

 segmentation are ably discussed in Dr. E. L. Mark's paper on 

 the Embryology of Limax, published by the Museum of Com- 

 parative Zoology at Cambridge, and in the later Biologie Cellu- 

 laire of Carnoy. The formation of the egg in the various 

 groups of the animal kingdom has been summarized by Lud- 

 wig in the first volume of Semper's " Arbeiten," and on this 

 subject one should also consult the various papers of the 

 younger Van Beneden. Less is known of the formation of 

 spermatozoa, but some useful resumes by Bloomfield occur in 

 various numbers of the Quarterly Journal of Microscopical 

 Science. Eol's papers on the early stages of the star-fish egg 

 occur in a Swiss journal; Balfour's abtract, however, will an- 

 swer all ordinary purposes. 



NEW METHODS OF WORK. 



BY ALBERT E. JENKINS, ANN ARBOR, MICH. 



A New Stain from the Hucldeherry. 



N Volume XXIII of the " Archiv fur Mikroskopishe Anato- 

 mie," Dr. Lardowsky describes a new stain, which he highly 

 recommends for the karyokinetic figurci and the cellulose walls 

 of plants. 



The fresh ripe fruit of the huckleberry, Vaccinum myrtil- 

 lus, is washed in water, and the juice expressed and mixed 



I 



