Ts 
Dr. Huntsman kindly arranged that fresh clams and sea water for this 
experiment be sent from St. Andrews, N.B. They arrived in perfect condition 
and were opened and prepared at once. 
and plated after 24 hours to determine purity. 
Transfers were made from all original stab cultures to beer peptone broth 
This having been established, 
tubes of series A.B.C. were seeded in triplicate from broth cultures of all organ- 
Three tubes of each series were kept uninoculated as controls. 
isms. 
No:] . Fe Fe Fe Sn Sn Sn Fe -Sn Fe Sn Fe Sn 
re) Gir) = re | T= (+) = = 
Sry tcte) te He a = = (=) GE) + 
Ge) tert: (+) Gir) a = 7 () “i ate 
AS sia) eal) C42) (aia) =e 5 qa =; (+) (+) | (4A) 
EROS) |r) CF) = =f + (+) (=F) (-B) 
1 er Cores a) (a) == = a= CAG) aise ae) 
NEE NGI) oot a = ~ ry, ae 25 sig 
16) + + + — = = = = 
LES Ge PS Gi) a) + a Tr (+) (4) a) 
20SEC) h(t) (i) 35 a at (=F) CH) (ra) 
26.) + Co) CE} + =e) a (+) (4p) (+e) 
ag] (+)|(+)| (4) | +] # ] +t (+) H) | 4) 
aL} CH) | + - + | - - - - ~ 
ge} CA) e | (+) os ig) =i me CE) GF) (+r) 
Reddish Reddish 
SOW): | (tz) ir) 17 =e ae ee i WES) (+) (ap? 
far) se) (cb) Cr) a r =e =i; ae a 
Ga) le CT) Ga vn ee 2: Uae) (+) (ea) 
15h) a a ro (+) oT a + Cr (+) = 
AN oo al fe Ou) (+) oe == = CH (+) (Ca) 
ips tC) (+) +t ae + (+) ‘Ce, (+) 
Seite alc (ie) Gy) + as Goce (+) (CP) (+) 
SAG) el (=e) (+) (=) 4) CE) = (-F) Gi) (se) 
15.110 Coir) ra Nek Get) Sr et (+) Car) D-P)tae) Gt) 
DON CS) eG) (+) i = + (+) (Gr) (+) 
AE cS aN Ge dl PA) ee) a a = at a as 
LBs (ce); ) Gt) + a = (sr) =aGe) (+) 
SUN CE Ck) (+) =e te =P (+) (Ce) = 
Fe Alt) CG) Gra) Cr) Zs (+) an (a) 
Reddish 
*Extra tubes. 
(+) indicates very deep blackening of all clam meat. 
+ indicates deep blackening of part of clam meat. 
+ indicates dark greenish-brown colour—more characteristic of tin sulphide. 
— indicates no blackening. 
The seeded clam tubes were left at room temperature until growth. was 
evident—24 to 48 hours—then kept in an unheated room from four to six weeks. 
result is seen in the accompanying table. 
Blackening developed rapidly in many nes slowly in others. 
The final 
Twenty-eight of the seventy-six original cultures were thus seen to be 
capable of producing blackening when provided with the elements to be found 
a 
